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Knockout Mouse Core Laboratory

Background

The Knockout Mouse Core Laboratory is primarily tasked with producing transgenic mice for intramural researchers. The staff also offers consultation in producing transgenic mice and numerous related specialty services, ranging from conceptual design of targeting schemes for conventional targeting to working with mouse embryonic stem cells to CRISPR/Cas9-mediated genome editing.

Services We Provide

  • Develop genome targeting strategy and vector construction
  • Electroporation of mouse embryonic stem cells with targeting vector and subsequent culturing and screening via PCR and/or Southern blot analysis
  • Microinjection of genetically modified ES-cells into mouse blastocysts to generate chimeras and their subsequent breeding to identify germline transmission
  • Culturing of user-provided genetically modified ES cells for QAL/QC, and subsequent blastocyst micro-injection
  • Single-cell embryo micro-injection of CRISPR/Cas9 system to generate NHEJ mutations and homologous repair mediated targeted genomic mutations
  • CRISPR/Cas9-assisted targeting in ES cells as well as other cell lines
  • Derivation of mouse ES cell lines from existing transgenic mice
  • Micro-injection of genetically modified ES cells into blastocysts for chimeric analysis
  • Cryo-recovery of frozen mouse embryos
  • Assist in troubleshooting problems with previously generated transgenic mouse lines
  • Long-term storage of frozen mouse embryos
  • In collaboration with CMB/ACUC, develop efficient breeding strategy to optimize animal use and minimizing the animal cost for the institute

NIEHS Shared and Core Facilities are available to NIH researchers. Information for staff on utilizing these services can be found on the NIEHS Junction or by contacting the staff below.

Scientific Staff

Manas Ray
Manas K. Ray, Ph.D.
Director, Knockout Mouse Core Laboratory
P.O. Box 12233
Mail Drop E4-02
Durham, N.C. 27709

Tel 919-316-4553
Fax 919-541-3800
ray6@niehs.nih.gov
Artiom Gruzdev, Ph.D.
Artiom Gruzdev, Ph.D.
Biologist
P.O. Box 12233
Mail Drop E4-02
Durham, N.C. 27709

Tel 919-541-5540
Fax 919-541-0696
artiom.gruzdev@nih.gov
Greg Scott
Gregory J. Scott
Biologist
P.O. Box 12233
Mail Drop E4-02
Durham, N.C. 27709

Tel 919-316-4635
Fax 919-541-3800
scott9@niehs.nih.gov
Thomas Hagler
Thomas B. Hagler
Fellow — IRTA Postbaccalaureate
P.O. Box 12233
Mail Drop E4-02
Durham, N.C. 27709

Tel 919-541-0343
thomas.hagler@nih.gov