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Developmental and Reproductive Toxicology Group

Chad R. Blystone, Ph.D.
Chad R. Blystone, Ph.D.
Toxicologist
Tel 984-287-3113
[email protected]
P.O. Box 12233
Mail Drop K2-13
Durham, NC 27709

Chad Blystone, Ph.D., D.A.B.T., is a toxicologist in the Toxicology Branch of the Division of Translational Toxicology (DTT) at the National Institute of Environmental Health Sciences. He serves as a study scientist for chemicals selected for toxicity and carcinogenicity evaluation by the NTP. Currently he is the lead on studies evaluating chemicals within the classes of parabens, phthalates, ethylene glycol ethers and perfluoroalkyl acids. These studies include in vitro and in vivo evaluation of toxicity, toxicokinetics, absorption, disposition, metabolism and excretion of the chemical. He has a particular interest in elucidating chemical toxicity to reproduction and development, developmental basis of disease, and the adverse effects from chemical mixtures.

Blystone received a B.S. in zoology from Auburn University, Auburn, Alabama, an M.S. in zoology from Miami University, Oxford, Ohio, and a Ph.D. in toxicology from North Carolina State University, Raleigh, North Carolina. He completed postdoctoral work at the National Health and Environmental Effects Research Laboratory within the U. S. Environmental Protection Agency, and at the NTP at the NIEHS. Blystone joined the NTP in 2009.

Selected Publications

  1. Dzierlenga AL, Robinson VG, Waidyanatha S, DeVito MJ, Eifrid MA, Gibbs ST, Granville CA, Blystone CR. Toxicokinetics of perfluorohexanoic acid (PFHxA), perfluorooctanoic acid (PFOA) and perfluorodecanoic acid (PFDA) in male and female Hsd:Sprague dawley SD rats following intravenous or gavage administration. Xenobiotica 2019 doi: 10.1080/00498254.2019.1683776 [Online 7 November 2019]. [Abstract Dzierlenga AL, Robinson VG, Waidyanatha S, DeVito MJ, Eifrid MA, Gibbs ST, Granville CA, Blystone CR. Toxicokinetics of perfluorohexanoic acid (PFHxA), perfluorooctanoic acid (PFOA) and perfluorodecanoic acid (PFDA) in male and female Hsd:Sprague dawley SD rats following intravenous or gavage administration. Xenobiotica 2019 doi: 10.1080/00498254.2019.1683776 [Online 7 November 2019].] 
  2. Huang MC, Robinson VG, Waidyanatha S, Dzierlenga AL, Devito MJ, Eifrid MA, Gibbs ST, Hong SP, Blystone CR. 2019. Toxicokinetics of 8:2 fluorotelomer alcohol (8:2-FTOH) in male and female Hsd:Sprague Dawley SD rats after intravenous and gavage administration. Toxicol Rep 6:924-932. [Abstract Huang MC, Robinson VG, Waidyanatha S, Dzierlenga AL, Devito MJ, Eifrid MA, Gibbs ST, Hong SP, Blystone CR. 2019. Toxicokinetics of 8:2 fluorotelomer alcohol (8:2-FTOH) in male and female Hsd:Sprague Dawley SD rats after intravenous and gavage administration. Toxicol Rep 6:924-932.]
  3. Huang MC, Dzierlenga AL, Robinson VG, Waidyanatha S, Devito MJ, Eifrid MA, Granville CA, Gibbs ST, Hong SP, Pirone JR, Blystone CR. Toxicokinetics of perfluorobutane sulfonate (PFBS), perfluorohexane-1-sulphonic acid (PFHxS), and perfluorooctane sulfonic acid (PFOS) in male and female Hsd:Sprague Dawley SD rats after intravenous and gavage administration. Toxicology Reports 2019 6():645-655. [Abstract Huang MC, Dzierlenga AL, Robinson VG, Waidyanatha S, Devito MJ, Eifrid MA, Granville CA, Gibbs ST, Hong SP, Pirone JR, Blystone CR. Toxicokinetics of perfluorobutane sulfonate (PFBS), perfluorohexane-1-sulphonic acid (PFHxS), and perfluorooctane sulfonic acid (PFOS) in male and female Hsd:Sprague Dawley SD rats after intravenous and gavage administration. Toxicology Reports 2019 6():645-655.] 
  4. Matthews JM, Brown SS, Patel PR, Black SR, Banks TT, Etheridge AS, Fennell TR, Snyder RW, Blystone CR, Waidyanatha S. Metabolism and disposition of [14C]n-butyl-p-hydroxybenzoate in male and female Harlan Sprague Dawley rats following oral administration and dermal application. Xenobiotica; the fate of foreign compounds in biological systems 2013 43(2):169-181. [Abstract Matthews JM, Brown SS, Patel PR, Black SR, Banks TT, Etheridge AS, Fennell TR, Snyder RW, Blystone CR, Waidyanatha S. Metabolism and disposition of [14C]n-butyl-p-hydroxybenzoate in male and female Harlan Sprague Dawley rats following oral administration and dermal application. Xenobiotica; the fate of foreign compounds in biological systems 2013 43(2):169-181.]
  5. Blystone CR, Elmore SA, Witt KL, Malarkey DE, Foster PMD. Toxicity and carcinogenicity of androstenedione in F344/N rats and B6C3F1 mice. Food and chemical toxicology 2011, 49(9):2116-2124. [Abstract Blystone CR, Elmore SA, Witt KL, Malarkey DE, Foster PMD. Toxicity and carcinogenicity of androstenedione in F344/N rats and B6C3F1 mice. Food and chemical toxicology 2011, 49(9):2116-2124.]
  6. Hotchkiss AK, Rider CV, Furr J, Howdeshell KL, Blystone CR, Wilson VS, Gray LE. In utero exposure to an AR antagonist plus an inhibitor of fetal testosterone synthesis induces cumulative effects on F1 male rats. Reproductive toxicology (Elmsford, N.Y.) 2010, 30(2):261-270. [Abstract Hotchkiss AK, Rider CV, Furr J, Howdeshell KL, Blystone CR, Wilson VS, Gray LE. In utero exposure to an AR antagonist plus an inhibitor of fetal testosterone synthesis induces cumulative effects on F1 male rats. Reproductive toxicology (Elmsford, N.Y.) 2010, 30(2):261-270.]
  7. Blystone CR, Bishop JB, Chapin RE, Kissling GE, Wolfe GW, Foster PMD. Determination of the di-(2-ethylhexyl) phthalate NOAEL for reproductive development in the rat: importance of the retention of extra animals to adulthood. Toxicological Sciences 2010, 116(2):640-646. [Abstract Blystone CR, Bishop JB, Chapin RE, Kissling GE, Wolfe GW, Foster PMD. Determination of the di-(2-ethylhexyl) phthalate NOAEL for reproductive development in the rat: importance of the retention of extra animals to adulthood. Toxicological Sciences 2010, 116(2):640-646.]
  8. Blystone CR, Lambright CS, Cardon MC, Furr J, Rider CV, Hartig PC, Wilson VS, and Gray LE Jr. Cumulative and antagonistic effects of a mixture of the antiandrogens vinclozolin and iprodione in the pubertal male rat. Toxicol Sci. 2009, Sept; 111(1) 179-188. [Abstract Blystone CR, Lambright CS, Cardon MC, Furr J, Rider CV, Hartig PC, Wilson VS, and Gray LE Jr. Cumulative and antagonistic effects of a mixture of the antiandrogens vinclozolin and iprodione in the pubertal male rat. Toxicol Sci. 2009, Sept; 111(1) 179-188.]
  9. Hotchkiss AK, Rider CV, Blystone CR, Wilson VS, Hartig PC, Ankley GT, Foster PM, Gray CL, and LE Gray, Jr. Fifteen years after “Wingspread” – Environmental Endocrine Disruptors and human and wildlife health: Where we are today and where we need to go. Toxicol Sci. 2008, Oct; 105(2): 235-259. [Abstract Hotchkiss AK, Rider CV, Blystone CR, Wilson VS, Hartig PC, Ankley GT, Foster PM, Gray CL, and LE Gray, Jr. Fifteen years after “Wingspread” – Environmental Endocrine Disruptors and human and wildlife health: Where we are today and where we need to go. Toxicol Sci. 2008, Oct; 105(2): 235-259.]
  10. Hartig PC, Cardon MC, Blystone CR, Gray LE Jr, and Wilson VS. High Throughput adjustable 96-well plate assay for androgen receptor binding: a practical approach for EDC screening using the chimpanzee AR. Toxicol Lett. 2008 Sept. 26; 181(2):126-131. [Abstract Hartig PC, Cardon MC, Blystone CR, Gray LE Jr, and Wilson VS. High Throughput adjustable 96-well plate assay for androgen receptor binding: a practical approach for EDC screening using the chimpanzee AR. Toxicol Lett. 2008 Sept. 26; 181(2):126-131.]
  11. Howdeshell KL, Wilson VS, Furr J, Lambright CR, Rider CV, Blystone CR, Hotchkiss AK, Gray LE Jr. A mixture of five phthalate esters inhibits fetal testicular testosterone production in the Sprague-dawley rat in a cumulative, dose-additive manner. Toxicol Sci. 2008, Sept.; 105(1): 153-165. [Abstract Howdeshell KL, Wilson VS, Furr J, Lambright CR, Rider CV, Blystone CR, Hotchkiss AK, Gray LE Jr. A mixture of five phthalate esters inhibits fetal testicular testosterone production in the Sprague-dawley rat in a cumulative, dose-additive manner. Toxicol Sci. 2008, Sept.; 105(1): 153-165.]
  12. Wilson VS, Blystone CR, Hotchkiss AK, Rider CV, and Gray LE, Jr. Diverse Mechanisms of Antiandrogen Action: Impact on Male Reproductive Tract Development. Int J Androl. 2008 Apr; 31(2):178-187. [Abstract Wilson VS, Blystone CR, Hotchkiss AK, Rider CV, and Gray LE, Jr. Diverse Mechanisms of Antiandrogen Action: Impact on Male Reproductive Tract Development. Int J Androl. 2008 Apr; 31(2):178-187.]
  13. Blystone CR, Lambright CS, Furr J, Wilson VS, and LE Gray Jr. Iprodione delays male rat pubertal development, reduces serum testosterone levels, and decreases ex vivo testicular testosterone production. Toxicol Lett. 2007, Nov 1; 174(1-3):74-81. [Abstract Blystone CR, Lambright CS, Furr J, Wilson VS, and LE Gray Jr. Iprodione delays male rat pubertal development, reduces serum testosterone levels, and decreases ex vivo testicular testosterone production. Toxicol Lett. 2007, Nov 1; 174(1-3):74-81.]
  14. Blystone CR, Lambright CS, Howdeshell KL, Furr J, Sternberg RM, Butterworth BC, Durhan EJ, Makynen EA, Ankley GT, Wilson VS, LeBlanc GA, and LE Gray Jr. Sensitivity of fetal rat testicular steroidogenesis to maternal prochloraz exposure and the underlying mechanism of inhibition. Toxicol Sci. 2007, Jun; 97(2):512-519. [Abstract Blystone CR, Lambright CS, Howdeshell KL, Furr J, Sternberg RM, Butterworth BC, Durhan EJ, Makynen EA, Ankley GT, Wilson VS, LeBlanc GA, and LE Gray Jr. Sensitivity of fetal rat testicular steroidogenesis to maternal prochloraz exposure and the underlying mechanism of inhibition. Toxicol Sci. 2007, Jun; 97(2):512-519.]
  15. Blystone CR, Furr J, Lambright CS, Howdeshell KS, Ryan BC, Wilson VS, LeBlanc GA, and LE Gray. Prochloraz strongly inhibits testosterone production at doses well below those that affect androgen-dependent organ weights or the onset of puberty in the male Sprague Dawley rat. Toxicol Sci. 2007 May; 97(1): 65-74. [Abstract Blystone CR, Furr J, Lambright CS, Howdeshell KS, Ryan BC, Wilson VS, LeBlanc GA, and LE Gray. Prochloraz strongly inhibits testosterone production at doses well below those that affect androgen-dependent organ weights or the onset of puberty in the male Sprague Dawley rat. Toxicol Sci. 2007 May; 97(1): 65-74.]
  16. Goetz AK, Ren H, Schmid JE, Blystone CR, Thillainadarajah I, Best DS, Nichols HP, Strader LF, Wolf DC, Narotsky MG, Rockett JC, Dix DJ. Disruption of Testosterone Homeostasis as a Mode of Action for the Reproductive Toxicity of Triazole Fungicides in the Male Rat. Toxicol Sci. 2007, Jan; 95(1):227-239 [Abstract Goetz AK, Ren H, Schmid JE, Blystone CR, Thillainadarajah I, Best DS, Nichols HP, Strader LF, Wolf DC, Narotsky MG, Rockett JC, Dix DJ. Disruption of Testosterone Homeostasis as a Mode of Action for the Reproductive Toxicity of Triazole Fungicides in the Male Rat. Toxicol Sci. 2007, Jan; 95(1):227-239]
  17. Rockett JC, Narotsky MG, Thompson KE, Thillainadarajah I, Blystone CR, Goetz AK, Ren H, Best DS, Murrell RN, Nichols HP, Schmid JE, Wolf DC, Dix DJ. Effect of conazole fungicides on reproductive development in the female rat. Reprod Toxicol. 2006, Nov; 22(4):647-658. [Abstract Rockett JC, Narotsky MG, Thompson KE, Thillainadarajah I, Blystone CR, Goetz AK, Ren H, Best DS, Murrell RN, Nichols HP, Schmid JE, Wolf DC, Dix DJ. Effect of conazole fungicides on reproductive development in the female rat. Reprod Toxicol. 2006, Nov; 22(4):647-658.]
  18. Tully DB, Bao W, Goetz AK, Blystone CR, Ren H, Schmid JE, Strader LF, Wood CR, Best DS, Narotsky MG, Wolf DC, Rockett JC, Dix DJ. Gene expression profiling in liver and testis of rats to characterize the toxicity of triazole fungicides. Toxicol Appl Pharmacol. 2006, Sep 15; 215(3):260-273. [Abstract Tully DB, Bao W, Goetz AK, Blystone CR, Ren H, Schmid JE, Strader LF, Wood CR, Best DS, Narotsky MG, Wolf DC, Rockett JC, Dix DJ. Gene expression profiling in liver and testis of rats to characterize the toxicity of triazole fungicides. Toxicol Appl Pharmacol. 2006, Sep 15; 215(3):260-273.]
  19. Woods TC, Blystone CR, Yoo J, and ER Edelman. Activation of EphB2 and its ligands promotes vascular smooth muscle cell proliferation. J Biol Chem. 2002, 277(3):1924-1927. [Abstract Woods TC, Blystone CR, Yoo J, and ER Edelman. Activation of EphB2 and its ligands promotes vascular smooth muscle cell proliferation. J Biol Chem. 2002, 277(3):1924-1927.]