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1.1 Training/Testing Set Validation Over a Single Run

  1. Double click on ExP.bat
  2. Click on Project and select Load Data

    screenshot of Expression Predictor software
  3. Select Input file

    screenshot of Expression Predictor software
  4. Click Open

    screenshot of Expression Predictor software
  5. Select the Training Set

    screenshot of Expression Predictor software
  6. Click on the "Insert>>" button on the Training Set Panel

    screenshot of Expression Predictor software
  7. Select the Testing Set

    screenshot of Expression Predictor software
  8. Click on the "Insert>>" button on the Testing Set Panel

    screenshot of Expression Predictor software
  9. Select a vigilance value from the slider

    screenshot of Expression Predictor software
  10. Click on the "Submit button

    screenshot of Expression Predictor software
  11. Click on the Prediction Tab to view the confusion matrix

    screenshot of Expression Predictor software
  12. Click on one of the numbers in the confusion matrix to view the list of samples that corresponds the prediction in the selected cell from the matrix

    screenshot of Expression Predictor software
    screenshot of Expression Predictor software

1.2 Training/Testing Set Validation over the Entire Vigilance Spectrum

  1. Repeat steps 1 to 8 from Section 1.1
  2. Click on the checkbox that says "Apply All Values in Vigilance Spectrum" 

    screenshot of Expression Predictor software
  3. Repeat steps 10 from Section 1.1
  4. Click on Prediction Tab to view the prediction results using vigilance values from 0 to 1 with 0.01 increment at each cycle 

    screenshot of Expression Predictor software
  5. Click on "Save As Text (Tab delimited)," enter the filename, and click Save 

    screenshot of Expression Predictor software

2.1 Leave One Out Cross Validation a Single Run

  1. Repeat steps 1 to 4 from Section 1.1
  2. Select Leave One Out Cross Validation 

    screenshot of Expression Predictor software
  3. Click on the Submit button 

    screenshot of Expression Predictor software
  4. Click on the Prediction Tab to view the confusion matrix 

    screenshot of Expression Predictor software
  5. Click on one of the numbers in the confusion matrix to view the list of samples that corresponds to the prediction in the selected cell from the matrix 

    screenshot of Expression Predictor software
    screenshot of Expression Predictor software

2.2 Leave One Out Cross Validation over the Entire Vigilance Spectrum

  1. Repeat steps 1 and 2 from Section 2.1
  2. Click on the checkbox that says “Apply All Values in Vigilance Spectrum” 

    screenshot of Expression Predictor software
  3. Repeat steps 3 Section 2.1
  4. Click on Prediction Tab to view the prediction results using vigilance values from 0 to 1 with 0.01 increment at each cycle 

    screenshot of Expression Predictor software
  5. Repeat step 5 from Section 1.2 to save prediction as tab-delimited text file.