In vivo murine models of disease:

• Lung fibrosis – bleomycin, silica, BHT
• Lung inflammation – LPS, cigarette smoke
• Pneumonia – bacteria, virus
• Asthma – House dust mite, OVA
• Systemic infection – L. monocytogenes
• Multiple sclerosis – EAE
• Cancer – B16F10 melanoma, MC38 adenocarcinoma
• In vivo methods – adoptive cell transfer, bone marrow chimeras, lineage tracing

In vitro/ex vivo molecular and cell biology methods:

• Cell culture
• Stable cell lines, siRNA/shRNA/CRISPR-CAS9
• Biochemistry – immunoblotting, immunoprecipitation
• scRNA-Seq, ATAC-Seq, ChIP-Seq
• Seahorse
• Metabolomic, proteomic analysis
• Confocal microscopy, super-resolution microscopy
• Flow cytometry, FACS
• Isolation and stimulation of human peripheral blood leukocytes