Clinical Investigation of Host Defense Group

Environmental Innate Immunity

Protein Discovery in Innate Immunity Signaling

  • Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
  • Affinity precipitation and immunoprecipitation
  • Immobilized metal affinity chromatography
  • Tandem mass spectrometry
  • Two-Dimensional Polyacrylamide Gel Electrophoresis

Role of Cholesterol Trafficking and Lipid Rafts in Innate Immunity

  • Raft isolation
  • Confocal microscopy
  • RNA interference
  • Cholesterol quantitation
  • Rho GTPase assays
  • Mouse models
  • Macrophage and neutrophil isolation