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Selected Publications

Pharmacogenetics Group

Nuclear Receptor CAR

  1. Hashiguchi T, Arakawa S, Takahashi S, Gonzalez FJ, Sueyoshi, T, Negishi m. Phosphorylation of Farnesoid X Receptor at Serine 154 Links Ligand Activation With Degradation. Molecular endocrinology (Baltimore, Md.) 2016 30(10):1070-1108. [Abstract]
  2. Hori T, Moore R, Negishi M. p38 MAP Kinase Links CAR Activation and Inactivation in the Nucleus via Phosphorylation at Threonine 38. Drug metabolism and disposition: the biological fate of chemicals 2016 44(6):871-876. [Abstract]
  3. Yasujima T, Saito K, Moore R, Negishi M. Phenobarbital and Insulin Reciprocate Activation of the Nuclear Receptor Constitutive Androstane Receptor through the Insulin Receptor. The Journal of pharmacology and experimental therapeutics 2016 357(2):367-374. [Abstract]
  4. Gotoh S, Negishi M. Statin-activated nuclear receptor PXR promotes SGK2 dephosphorylation by scaffolding PP2C to induce hepatic gluconeogenesis. Scientific reports 2015 5():14076-14076. [Abstract]
  5. Kodama S, Negishi M. Pregnane X Receptor Represses HNF4α Gene to Induce Insulin-Like Growth Factor-Binding Protein IGFBP1 that Alters Morphology of and Migrates HepG2 Cells. Molecular pharmacology 2015 88(4):746-757. [Abstract]
  6. Ohno M, Kanayama T, Moore R, Ray M, Negishi M. The Roles of Co-Chaperone CCRP/DNAJC7 in Cyp2b10 Gene Activation and Steatosis Development in Mouse Livers. PloS one 2014 9(12):e115663-. [Abstract]
  7. Gotoh S, Negishi M. Serum- and glucocorticoid-regulated kinase 2 determines drug-activated pregnane X receptor to induce gluconeogenesis in human liver cells. The Journal of pharmacology and experimental therapeutics 2014 348(1):131-140. [Abstract]
  8. Sueyoshi T, Li L, Wang H, Moore R, Kodavanti PR, Lehmler HJ, Negishi M, Birnbaum LS. Flame Retardant BDE-47 Effectively Activates Nuclear Receptor CAR in Human Primary Hepatocytes. Toxicological sciences: an official journal of the Society of Toxicology 2014 137(2):292-302. [Abstract]
  9. Mutoh S, Sobhany M, Moore R, Perera L, Pedersen L, Sueyoshi T, Negishi M. Phenobarbital indirectly activates the constitutive active androstane receptor (CAR) by inhibition of epidermal growth factor receptor signaling. Science signaling 2013 6(274):ra31-. [Abstract]
  10. Kodama S, Negishi M. Sulfotransferase genes: regulation by nuclear receptors in response to xeno/endo-biotics. Drug metabolism reviews 2013 45(4):441-449. [Abstract]
  11. Saito K, Moore R, Negishi M. p38 Mitogen-activated protein kinase regulates nuclear receptor CAR that activates the CYP2B6 gene. Drug metabolism and disposition: the biological fate of chemicals 2013 41(6):1170-1173. [Abstract ]
  12. Shindo S, Moore R, Flake G, Negishi M. Serine 216 phosphorylation of estrogen receptor α in neutrophils: migration and infiltration into the mouse uterus. PloS one 2013 8(12):e84462-. [Abstract]
  13. Saito K, Moore R, Negishi M. Nuclear receptor CAR specifically activates the two-pore K+ channel Kcnk1 gene in male mouse livers, which attenuates phenobarbital-induced hepatic hyperplasia. Toxicological sciences: an official journal of the Society of Toxicology 2013 132(1):151-161. [Abstract]
  14. Sobhany M, Kakuta Y, Sugiura N, Kimata K, Negishi M. The structural basis for a coordinated reaction catalyzed by a bifunctional glycosyltransferase in chondroitin biosynthesis. The Journal of biological chemistry 2012 287(43):36022-36028. [Abstract]
  15. Kamino H, Negishi M. The nuclear receptor constitutive active/androstane receptor (CAR) arrests DNA-damaged human hepatocellular carcinoma Huh7 cells at the G2/M phase. Molecular carcinogenesis 2012 51(2):206-12. [Abstract]
  16. Kodama, S., Hosseinpour, F., Goldstein, J.A., and Negishi, M. Liganded pregnane X receptor represses the human sulfotransferase SULT1E1 promoter through disrupting its chromatin structure. Nucleic Acids Research 2011 39(19):8392-8403. [Abstract]
  17. Osabe, M., Negishi, M. Active ERK1/2 protein interacts with the phosphorylated nuclear constitutive active/androstane receptor (CAR; NR1I3), repressing dephosphorylation and sequestering CAR in the cytoplasm. The Journal of biological chemistry 2011 286(41):35763-35769. [Abstract]
  18. Yamazaki, Y., Moore, R., Negishi, M. Nuclear receptor CAR (NR1I3) is essential for DDC-induced liver injury and oval cell proliferation in mouse liver. Lab Invest 2011 91(11):1624-1633. [Abstract]
  19. Kamino, H., Moore, R., and Negishi, M. The role of a novel CAR-induced gene TUBA8 in hepatocellular carcinoma cell lines. Cancer genetics 2011 204(7):382-391. [Abstract]  
  20. Sueyoshi, T., Green, W.D., Vinal, K., Woodrum, T.S., Moore, R., and Negishi, M. Garlic extract diallyl sulfide (DAS) activates nuclear receptor CAR to induce the Sult1e1 gene in mouse liver. PLOS ONE 2011 6(6):e21229-. [Abstract]  
  21. Kodama, S., and Negishi, M.: Pregnane X Receptor PXR Activates the GADD45{beta} Gene, Eliciting the p38 MAPK Signal and Cell Migration. The Journal of biological chemistry 2011 286(5):3570-3578. [Abstract]
  22. Kamino, H., Yamazaki, Y., Saito, K., Takizawa, D., Kakizaki, S., Moore, R., and Negishi, M.: Nuclear receptor CAR-regulated expression of the FAM84A gene during the development of mouse liver tumors. International Journal of Oncology 2011 38(6):1511-1520. [Abstract]
  23. Yao, R., Yasuoka, A., Kamei, A., Kitagawa, Y., Tateishi, N., Tsuruoka, N., Kiso, Y., Sueyoshi, T., Negishi, M., Misaka, T., and Abe, K.: Dietary flavonoids activate constitutive androstane receptor, CAR. Journal of agricultural and food chemistry 2010 58(4):2168-2173. [Abstract]
  24. Konno, Y., Kamino, H., Moore, R., Lih, F., Tomer, K.B., Zeldin, D.C., Goldstein, J.A., and Negishi, M.: The nuclear receptors constitutive active/androstane receptor and pregnane x receptor activate the Cyp2c55 gene in mouse liver. Drug Metabolism and Disposition 2010 38(7):1177-1182. [Abstract]
  25. Yamamoto, Y., Moore, R., Flavell, R.A., Lu, B., and Negishi, M.: Nuclear receptor CAR represses TNFalpha-induced cell death by interacting with the anti-apoptotic GADD45B. PloS one 2010 5(4):e10121. [Abstract]
  26. Konno, Y., Kodama, S., Moore, R., Kamiya, N., and Negishi, M.: Nuclear xenobiotic receptor pregnane X receptor locks corepressor silencing mediator for retinoid and thyroid hormone receptors (SMRT) onto the CYP24A1 promoter to attenuate vitamin D3 activation. Molecular pharmacology 2009 75(2):265-271. [Abstract]
  27. Yeung, E.Y., Sueyoshi, T., Negishi, M., and Chang, T.K.: Identification of ginkgo biloba as a novel activator of pregnane X receptor. Drug metabolism and disposition: the biological fate of chemicals 2008 36(11):2270-2276. [Abstract]
  28. Sobhany M, Kakuta Y, Sugiura N, Kimata K, Negishi M.: The chondroitin polymerase K4CP and the molecular mechanism of selective bindings of donor substrates to two active sites. The Journal of biological chemistry 2008 283(47):32328-32333. [Abstract]
  29. Yamamoto, Y., and Negishi, M.: The antiapoptotic factor growth arrest and DNA-damage-inducible 45 beta regulates the nuclear receptor constitutive active/androstane receptor-mediated transcription. Drug metabolism and disposition: the biological fate of chemicals 2008 36(7):1189-1193. [Abstract]
  30. Li, L., Chen, T., Stanton, J.D., Sueyoshi, T., Negishi, M., and Wang, H.: The peripheral benzodiazepine receptor ligand 1-(2-chlorophenyl-methylpropyl)-3-isoquinoline-carboxamide is a novel antagonist of human constitutive androstane receptor. Mol. Pharmacol. 2008 74(2):443-453. [Abstract]
  31. Konno, Y., Negishi, M., and Kodama, S.: The roles of nuclear receptors CAR and PXR in hepatic energy metabolism. Drug metabolism and pharmacokinetics 2008 23(1):8-13. [Abstract]
  32. Inoue, K.: Nuclear receptor CAR requires early growth response 1 to activate the human cytochrome P450 gene. The Journal of biological chemistry 2008 283(16):10425-10432. [Abstract]
  33. Hosseinpour, F., Timsit, Y., Koike, C., Matsui, K., Yamamoto, Y., Moore, R., and Negishi, M.: Overexpression of the Rho-guanine nucleotide exchange fractor ECT2 inhibits nuclear translocation of nuclear receptor CAR in the mouse liver. FEBS letters 581(25):4937-4942, 2007. [Abstract]
  34. Kodama, S., Moore, R., Yamamoto, Y., and Negishi, M.: Human nuclear pregnane X receptor cross-talk with CREB to repress cAMP activation of the glucose-6-phosphatase gene. Biochem J 407:373–381, 2007. [Abstract]
  35. Tien, E., Matsui, K., Moore, R., and Negishi, M.: The nuclear receptor constitutively active/androstane receptor regulates type 1 deiodinase and thyroid hormone activity in regenerating liver. J. Pharmacol. Exp. Ther. 320: 307-313, 2007. [Abstract]
  36. Yamamoto, Y., Moore, R., Goldsworthy, T. L., Negishi, M., and Maronpot, R. R.: The orphan nuclear receptor constitutive active/androstane receptor is essential for liver tumor promotion by phenobarbital in mice. Can. Res. 64: 7197-7200, 2004. [Abstract]
  37. Kodama, S., Koike, C., Negishi, M., and Yamamoto, Y.: Nuclear receptors CAR and PXR cross talk with FOXO1 to regulate genes that encode drug metabolizing and gluconeogenic enzymes. Mol. Cell. Biol. 24: 7931-7940, 2004. [Abstract]
  38. Kobayashi, K., Sueyoshi, T., Inoue, K., Moore, R., and Negishi, M.: Cytoplasmic accumulation of the nuclear receptor CAR by a tetratricopeptide repeat protein in HepG2 cells. Mol. Pharmacol. 64: 1069-1075, 2003. [Abstract]
  39. Ueda, A., Hamadeh, H. K., Webb, H.K., Yamamoto, Y., Sueyoshi, T., Afshari, C.A., Lehmann, J., and Negishi, M.: Diverse roles of the nuclear orphan receptor CAR in regulating hepatic genes in response to phenobarbital. Mol. Pharmacol. 61: 1-6, 2002. [Abstract]
  40. Kawamoto, T., Sueyoshi, T., Zelko, T., and Negishi, M.: Phenobarbital-responsive nuclear translocation of the receptor CAR in induction of the CYP2B gene. Mol. Cell. Biol.19: 6318-6322, 1999. [Abstract]
  41. Sueyoshi, T., Kawamoto, T., Zelko, I., Honkakoski, P., and Negishi, M.: The repressed nuclear receptor CAR responds to phenobarbital in activating the human CYP2B6 gene. J. Biol. Chem. 274: 6043-6046, 1999. [Abstract]
  42. Honkakoski, P., Zelko, I., Sueyoshi, T., and Negishi, M.: The nuclear receptor CAR-retinoid X receptor heterodimer activates the phenobarbital-responsive enhancer module of the CYP2B gene. Mol. Cell. Biol.18: 5652-5658, 1998. [Abstract]

Molecular Glycobiology

  1. Sobhany M, Kakuta Y, Sugiura N, Kimata K, Negishi M.: The chondroitin polymerase K4CP and the molecular mechanism of selective bindings of donor substrates to two active sites. The Journal of biological chemistry 2008 283(47):32328-32333. [Abstract]
  2. Sobhany, M., Dong, J., and Negishi, M.: Two-step mechanism that determines the donor binding specificity of human N-acetylhexosaminyltransferase. J. Biol. Chem. 280: 23441-23445, 2005. [Abstract]
  3. Pedersen, L.C., Dong, J., Taniguchi, F., Kitagawa, H., Krahn, J., Pedersen, L.G., Sugahara, K., and Negishi, M.: Crystal structure of an alpha 1, 4-N-acetylhexosaminyltransferase (EXTL2), a member of the exostosin gene family involved in heparan sulfate biosynthesis. J. Biol. Chem. 278: 14420-14428, 2003. [Abstract]
  4. Pedersen, L.C., Darden, T.A., and Negishi, M.: Crystal structure of beta 1,3-glucuronyltransferase I in complex with active donor substrate UDP-GlcUA. J. Biol. Chem. 277: 21869-21873, 2002. [Abstract]