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Fluorescence Microscopy and Imaging Center

Services

The Fluorescence Microscopy and Imaging Center provides intramural researchers with access to a variety of advanced imaging equipment and techniques that will further their research. The center’s experienced staff also offers training on image acquisition and data analysis.

Background

Mouse kidney capillaries, known as glomerulus, labeled with Alexa 488, Alexa 594,a and DAPI
Mouse kidney capillaries, known as glomerulus, labeled with Alexa 488, Alexa 594,a and DAPI.

What is fluorescence microscopy?

Fluorescence microscopy is a non-destructive way of tracking or analyzing tissues, cells, or cellular structures. The procedure includes labeling an antibody with a fluorescent dye known as a fluorophore, and allowing it to find its target antigen within a sample. Fluorophores may also be covalently bonded to small molecules or proteins. Samples are visualized using an array of microscopic methods.

Types of equipment we use:

  • Zeiss LSM 880 confocal microscope with Airy-scan
  • Zeiss LSM 780 confocal microscope
  • Zeiss LSM 710 confocal and multiphoton microscope with
  • Zeiss LSM 510 nlo upright confocal and multiphoton microscope
  • Olympus TIRF microscopes
  • Olympus and Zeiss epifluorescence microscopes
  • Image Analysis workstations

NIEHS Shared and Core Facilities are available to NIH researchers. Information for staff on utilizing these services may be found on the NIEHS Junction or by contacting the staff below.

Scientific Staff

Jeff Tucker
Charles J. Tucker
Director, Fluorescence Microscopy and Imaging Center
P.O. Box 12233
Mail Drop F2-09
Durham, N.C. 27709

Tel 919-541-7656
tucker1@niehs.nih.gov
Agnus Janoshazi
Agnes K. Janoshazi, Ph.D.
Biophysicist
P.O. Box 12233
Mail Drop F2-09
Durham, N.C. 27709

Tel 919-541-1542
agnes.janoshazi@nih.gov
Erica Scappini, M.S.
Erica Scappini, M.S.
Biologist
P.O. Box 12233
Mail Drop F2-09
Durham, N.C. 27709

Tel 919-541-5318
Fax 919-541-4611
scappinie@niehs.nih.gov