Expanding Genome Integrity Assays to Population Studies
This list is grantees funded through the Expanding Genome Integrity Assays to Population Studies (U01) Research Project Cooperative Agreement (RFA-ES-17-006).
The cadre of supported projects both develop novel technologies and continue to improve on existing ones. Among the former are assays that have been largely tested in laboratory settings but would be paired with small to moderately sized population studies. Many of the applications address a central need of comparing measures of activity in low-invasive and accessible biosamples, such as blood or buccal samples, with activity in the target tissues (i.e., human bronchial, skin, or lung) Several of the projects devote effort to developing assays based on the widely-used comet assay, which has recently been advanced to a high-throughput chip and is commercially available. These aims are paired with novel assays that promise to measure individual DNA repair pathways (Base-Excision Repair, Nucleotide-Excision Repair, Non-Homologous End Joining, MisMatch Repair) as well as Global Genomic Repair to determine the best measures for the collaborating population studies. All the assays are set for pilot testing in human cohorts ranging from several dozen to hundreds of subjects and cases that evaluate cancer of the lung, breast, and skin; chronic obstructive pulmonary disease; neurodegeneration; progeria; and xeroderma pigmentosum.
Albert Einstein College of Medicine
Assessing genome sequence integrity in normal human cells
Principal Investigators: Jan Vijg, Ph.D., Simon Spivack, M.D.
DNA Repair Phenotype the Missing Link in Breast Cancer Risk Assessment
Principal Investigator: Mary Beth Terry, Ph.D.
Fred Hutchinson Cancer Research Center
PREcision Disease preventIon via somatiC muTagenesis enumeration (PREDICTION)
Principal Investigator: Jason Bielas, Ph.D.
Multi-Pathway DNA Repair Capacity Measurements in Lung Cancer Patients & Healthy Controls
Principal Investigators: Zachary Nagel, Ph.D. (Harvard), and David Christiani, M.D., Ph.D. (Harvard), Bevin Engelward, Ph.D. (MIT)
Lovelace Biomedical & Environmental Research Institute
DNA Repair Capacity Assays for Lung Disease Risk Assessment
Principal Investigator: Steven A Belinsky, M.D., Ph.D.
University of Minnesota
Measuring Nucleotide Excision Repair in Human Populations
Principal Investigator: Laura Niedernhofer, Ph.D.
University of South Alabama
Measuring genomic DNA damage and DNA repair capacity in longitudinal population samples - a step towards precision prevention
Principal Investigator: Robert Sobol, Ph.D.