The Viral Vector Core (VVC) was established in 2007 as part of the Neurobiology Laboratory to promote the safe and effective use of viruses for all NIEHS investigators. The core provides consultation in development, production, and validation of new viral vectors for gene delivery and aids Principal Investigators (PIs) in troubleshooting viral applications.
Viral vectors are a highly efficient tool for delivering genes into cells. They can be used to efficiently generate stable cell lines, to uniformly transduce primary cell cultures for biochemical studies, and to transduce tissues in vitro or in vivo. They also hold the potential for reducing the time and cost of generating transgenic animals.
The mission of the core is to provide NIEHS investigators with quality viral products and expert advice to facilitate research. The core achieves its mission by importing and implementing the latest viral technologies, providing NIEHS with efficient and cost effective ways of utilizing viruses in research, importing and implementing the latest viral technology, ensuring accountability and compliance, sponsoring safety, and providing training for viral use.
Service to the Institutes
The core offers several types of vectors for gene delivery and use in research including: Adeno-associated virus (AAV), Adenovirus (Ad5), Influenza virus, Lentivirus, Lymphocytic choriomeningitis virus (LCMV), Rabies delta-G virus, Respiratory syncytial virus (RSV), Retrovirus, Scale drop disease virus (SDDV), Sindbis virus, and Zika virus.
Each vector has different strengths and weaknesses. We encourage users to consult the core staff about the most appropriate choice for their experiments.
- Consultation in viral vectors applications
- Production and titration of viruses
- Transduction and creation of stable cell lines
- Immortalizing primary cell lines
- Provision of ready-made viruses
- Consultation in molecular cloning and plasmid construction
- DNA plasmid preparation
- Training in viral applications and safe handling of viruses
NIEHS Shared and Core Facilities are available to NIH researchers. Information for staff on utilizing these services may be found on the NIEHS Junction or by contacting the staff below.
Negin P. Martin, Ph.D.
Director, Viral Vector Core
P.O. Box 12233Mail Drop F1-04Durham, N.C. 27709
David Chen, Ph.D.
111 Tw Alexander Dr
David P Rall Building
Research Triangle Park, NC 27709