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Viral Vector Core Laboratory

Viral Vector Core

Services

The Viral Vector Core (VVC) was established in 2007 as part of the Neurobiology Laboratory to promote the safe and effective use of viruses for all NIEHS investigators. The core provides consultation in development, production, and validation of viral vectors for gene delivery and aids Principal Investigators (PIs) in troubleshooting viral applications. The Viral Vector Core is also a teaching laboratory for training students and staff.

The core offers several types of vectors for gene delivery including: Adeno-Associated Virus (AAV), Lentivirus, Retrovirus, Adenovirus, Sindbis Virus, Rabies delta-G Virus, Respiratory Syncytial Virus (RSV) and Zika Virus.

Background

Viruses are highly efficient vehicles for delivering genes into cells, and can be used to efficiently generate stable cell lines, uniformly transduce primary cell cultures for biochemical studies, and transduce tissues in vitro or in vivo. Using viruses in vivo also complements and expands the generation and uses of transgenic animals and technology. Each vector has different strengths and weaknesses. We encourage users to consult the core staff about the most appropriate choice for their experiments.

Types of projects we do utilizing viral vectors:

  • Production and titration of viruses
  • Efficient and stable gene expression
  • Inducible gene expression
  • Gene knockdown by short hairpin RNA
  • Gene editing with CRISPR/Cas9
  • Creation of stable cell lines
  • Provision of ready-made viruses
  • Consultation in molecular cloning and plasmid construction
  • Consultation in viruses and viral vectors application

NIEHS Shared and Core Facilities are available to NIH researchers. Information for staff on utilizing these services may be found on the NIEHS Junction or by contacting the staff below.

Scientific Staff:

Martin, Negin
Negin P. Martin, Ph.D.
Acting Director, Viral Vector Core Laboratory
P.O. Box 12233
Mail Drop F1-04
Durham, N.C. 27709

Tel 919-541-7751
Fax 919-541-4611
martin12@niehs.nih.gov
Mitzie Walker
Mitzie L. Walker
Biologist
P.O. Box 12233
Mail Drop F1-04
Durham, N.C. 27709

Tel 919-541-2252
Fax 919-541-4611
walker7@niehs.nih.gov
Shih-Heng (David) Chen
David Chen, Ph.D.
Contractor — Scientist
Tel 984-287-3441
chens3@niehs.nih.gov
111 T W Alexander Dr
Rall Building
Research Triangle Park, NC 27709