Epigenomics and DNA Sequencing Core Facility

Services

The Epigenomics and DNA Sequencing Core Facility managed by Greg Solomon, Ph.D. provides NextGen Sequencing (NGS) and Sanger Sequencing services to researchers throughout the NIEHS community.

Background

Types of projects we do:

Whole Genome Sequencing (WGS) or Whole Genome Bisulphite Sequencing (WGBS) – analysis of DNA and DNA methylation in any genome, from microbes to humans
Whole Exome Sequencing (WES) – identification of coding variants
Targeted re-sequencing – identification of variants in genomic regions of interest in a cost-effective manner
RNA Sequencing (RNA-seq) – assesses gene expression, transcription, and RNA processing events in selected cells and tissues
Single Cell RNA Sequencing (scRNA-seq) – single cell analysis
Chromatin Immunoprecipitation Sequencing (ChIP-seq) – determines genomic occupancy of transcription factors and other chromatin-associated proteins
Assay for Transposase-Accessible Chromatin using Sequencing (ATAC-seq) – examines chromatin accessibility

Run Formats:

Single end – reads from only one end of the strand. Typical run lengths are between 36bp to 150bp. We can do as large as 300bp for special samples. Samples can be multiplexed from 2 to 96 samples per flow cell depending on the coverage needed and flow cell type used.
Paired end – reads from both ends of the strand. Typical read length is between 75bp and 150bp. We can do as large as 250bp for special samples. Samples can be multiplexed from 2 to 96 samples per flow cell depending on the coverage needed and flow cell type used.

Specialized Equipment:

NextSeq 500 NGS system
Two MiSeq NGS systems
NovaSeq 6000 NGS system
Two Agilent 2100 Bioanalyzers
Covaris S220 Focused-Ultrasonicator with Polyscience MM7 chiller
Applied Biosystems QuantStudio 6 Flex Real-Time PCR System
10x Genomics Chromium
Bio-Rad ddSeq

NIEHS Shared and Core Facilities are available to NIH researchers. Information for staff on utilizing these services can be found on the NIEHS Junction or by contacting the staff below.