About the Platform

We offer a targeted lipidomics method as our preferred tool for investigating lipids in disease-associated systems. This broad targeted approach leverages a conserved chemical logic of glycerolipids, sphingolipids, and sterols to measure relative levels of specific lipid molecules. We work with investigators to reach a clear, directed interpretation of the data. While relative quantitation often provides adequate information to resolve most lipid hypotheses, there is the possibility for pseudo-absolute quantification (quantification based on similarity to internal standards) as well as customization of the panel. Please indicate in the project request form the level of quantification (e.g., relative vs. pseudo-absolute) required and details can be discussed during consultation.

• Measurement of 1750 individual lipids.
• Acyl-chain resolution (i.e., length, number of double bonds) of phosphatidylethanolamine, phosphatidylcholine, sphingomyelin, ceramide, hexosylceramide, lactosylceramide, dihydroceramide, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, Ether-PE, triacylglycerol, diacylglycerol, monoacylglycerol, cholesterol esters, free fatty acids, and lysophospholipids.
• Compatibility with a wide range of biological matrices.

Cost

Please consult with the NIAID Core Director for cost information.

References

• Foliaki ST, Smith A, Schwarz B, Bohrnsen E, Bosio CM, Williams K, Orrú CD, Lachenauer H, Groveman BR, Haigh CL. 2023. Altered energy metabolism in Fatal Familial Insomnia cerebral organoids is associated with astrogliosis and neuronal dysfunction. PLoS Genet. 2023 Jan 19;19(1):e1010565. doi: 10.1371/journal.pgen.1010565. [ Abstract Foliaki ST, Smith A, Schwarz B, Bohrnsen E, Bosio CM, Williams K, Orrú CD, Lachenauer H, Groveman BR, Haigh CL. 2023. Altered energy metabolism in Fatal Familial Insomnia cerebral organoids is associated with astrogliosis and neuronal dysfunction. PLoS Genet. 2023 Jan 19;19(1):e1010565. doi: 10.1371/journal.pgen.1010565. ]
• Schwarz B, Sharma L, Roberts L, Peng X, Bermejo S, Leighton I, Casanovas-Massana A, Minasyan M, Farhadian S, Ko AI; Yale IMPACT Team; Dela Cruz CS, Bosio CM. 2021. Cutting Edge: Severe SARS-CoV-2 Infection in Humans Is Defined by a Shift in the Serum Lipidome, Resulting in Dysregulation of Eicosanoid Immune Mediators. J Immunol. 2021 Jan 15;206(2):329-334. doi: 10.4049/jimmunol.2001025. Epub 2020 Dec 4. [ Abstract Schwarz B, Sharma L, Roberts L, Peng X, Bermejo S, Leighton I, Casanovas-Massana A, Minasyan M, Farhadian S, Ko AI; Yale IMPACT Team; Dela Cruz CS, Bosio CM. 2021. Cutting Edge: Severe SARS-CoV-2 Infection in Humans Is Defined by a Shift in the Serum Lipidome, Resulting in Dysregulation of Eicosanoid Immune Mediators. J Immunol. 2021 Jan 15;206(2):329-334. doi: 10.4049/jimmunol.2001025. Epub 2020 Dec 4. ]
• Roberts LM, Schwarz B, Speranza E, Leighton I, Wehrly T, Best S, Bosio CM. 2021. Pulmonary infection induces persistent, pathogen-specific lipidomic changes influencing trained immunity. iScience. 2021 Aug 24;24(9):103025. doi: 10.1016/j.isci.2021.103025. [ Abstract Roberts LM, Schwarz B, Speranza E, Leighton I, Wehrly T, Best S, Bosio CM. 2021. Pulmonary infection induces persistent, pathogen-specific lipidomic changes influencing trained immunity. iScience. 2021 Aug 24;24(9):103025. doi: 10.1016/j.isci.2021.103025. ]

Contact

• Project requests should be directed to the Trans-NIH Metabolomics Core.
• Questions about this offering should be directed to Benjamin (Benji) Schwarz, Ph.D.

Benji Schwarz, Ph.D.
Staff Scientist

Tel 406-363-9285
[email protected]