Extraction of High Quality RNA

Studies requiring gene expression analysis by microarray methods require the extraction of high quality RNA. Fresh, frozen tissue is the optimal sample for these studies. This collection of webpages provide detailed protocols to assist the investigator in tissue collection, RNA extraction and RNA quality assessment. While techniques are demonstrated using the rat liver, the basic principles may be applied to most tissues regardless of species.

• Disruption and Homogenization of Tissue for the Extraction of RNA
• Instrument Cleaning - Rnase Decontamination
• RNA Isolation from Pico-scale samples (1 cell (10-25pg) - 10mg tissue) with the PicoPure
• RNA Isolation Using The Qiagen RNEasy Midi Kit
• Supplies