Clinical Investigation of Host Defense Group
Protein Discovery in Innate Immunity Signaling
- Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
- Affinity precipitation and immunoprecipitation
- Immobilized metal affinity chromatography
- Tandem mass spectrometry
- Two-Dimensional Polyacrylamide Gel Electrophoresis
Role of Cholesterol Trafficking and Lipid Rafts in Innate Immunity
- Raft isolation
- Confocal microscopy
- RNA interference
- Cholesterol quantitation
- Rho GTPase assays
- Mouse models
- Macrophage and neutrophil isolation
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