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Equipment

Immunohistochemistry

Much of the work carried out by DTT is in support of the National Toxicology Program (NTP), an interagency partnership of the Food and Drug Administration, National Institute for Occupational Safety and Health, and NIEHS.

Visit the NTP Website

The Immunohistochemistry (IHC) Laboratory utilizes two automated staining systems:

  • The flexible Biocare intelliPATH™ FLX is an open platform system capable of supporting multiple users and staining 50 slides simultaneously.
  • The fully automated Ventana Discovery™ XT is a start to finish system capable of staining 30 slides at a time.

The Laboratory uses modern procedures for standardized antigen retrieval, including the high capacity Biocare Decloaking Chamber™ NxGen.

A Leica CM3050S cryostat is used for sectioning frozen tissues.

A Reichert-Jung 2030 microtome is used for sectioning in-house control tissues.

The Laboratory also has a 10-head Olympus BX51 microscope with large screen viewing and image capture capabilities, and an Olympus BX61 microscope with motorized stage for viewing and capturing fluorescent stains.

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