2018 News

SRP PFAS Research Highlighted at Federal Remediation Technologies Roundtable

FRTR builds collaboration among federal agencies involved in hazardous waste site cleanup, including the U.S. Department of Defense, the U.S. Department of Energy, the U.S. Department of the Interior, the U.S. Environmental Protection Agency, the National Aeronautics and Space Administration, the Nuclear Regulatory Commission, and the National Institutes of Health.
(Photo courtesy of FRTR)

Superfund Research Program (SRP)-funded research related to per- and polyfluoroalkyl substances (PFAS) was highlighted at the November 7 Federal Remediation Technologies Roundtable (FRTR) meeting in Reston, Virginia. The goal of the meeting was to identify and discuss current technologies and the emerging science behind PFAS characterization and cleanup.

SRP Health Scientist Administrator Heather Henry, Ph.D., participated in a panel discussion about the research gaps and next steps for characterizing and cleaning up PFAS. Henry discussed the wide-ranging research that SRP-funded researchers are doing related to PFAS.

For example, she highlighted the new University of Rhode Island SRP Center, led by Rainer Lohmann, Ph.D., which is focused on understanding the sources, exposure, and health effects of PFAS. Henry described their novel passive sampling tools to detect and quantify PFAS in the air and water, as well as their efforts to understand the fate and transport of PFAS in the environment.

"The FRTR meeting was a great opportunity to share the important work SRP-funded researchers are doing related to PFAS," noted Henry. "In fact, many of the presentations by our federal partner agencies cited work from our grantees, reinforcing the good work they are doing in this emerging area."

Henry discussed innovative SRP-funded research on PFAS.
(Photo courtesy of Heather Henry)

Other highlighted SRP-funded research included:

• Research by David Sedlak, Ph.D., and Lisa Alvarez-Cohen, Ph.D., of the University of California, Berkeley SRP Center on the biotransformation of PFAS.
• Novel energy-efficient nanoreactors developed by Stephen Boyd, Ph.D., of the Michigan State University SRP Center that break the carbon-fluorine bond to de-fluorinate PFAS compounds.
• Cutting-edge cleanup technologies developed by SRP-funded small businesses EnChem Engineering Inc., CycloPure Inc., and Lynntech Inc.
• Human health and exposure research led by the University of Rhode Island and Brown University SRP Centers.

Sue Fenton, Ph.D., from the National Toxicology Program also attended the meeting and gave a presentation about the evaluation of the potential health effects of PFAS.

SRP Researchers and Trainees Travel to China for IEBMC Meeting

Carlin and Cormier pictured with four students who were awarded SRP travel grants to attend the IEBMC meeting. From left, Carlin; Natalie Fuentes from Pennsylvania State University; Emma Bowers, Ph.D., from the University of Arizona; Cormier; Chuqi Guo from Louisiana State University; and Hadi Abou El Hassan from the American University of Beirut.
(Photo courtesy of Danielle Carlin)

Superfund Research Program (SRP) grantees were well represented at the sixth annual International Experimental Biology and Medicine Conference (IEBMC), held October 19 – 21 in Chengdu, China. Co-sponsored by SRP, the 2018 IEBMC focused on environmental health and medicine.

IEBMC brings together international partners to advance experimental biology and medicine and facilitates conversation between established career scientists, policy officials, and trainees. Invited lecturers included current SRP grantees Stephania Cormier, Ph.D., from the Louisiana State University SRP Center and Robert Tanguay, Ph.D., of the Oregon State University SRP Center. Former SRP grantees Kenneth Ramos, Ph.D., of the University of Arizona and Alvaro Puga, Ph.D., of the University of Cincinnati were also invited lecturers.

Participants shared and discussed the latest research on air pollution and human health, assessing the toxicity of hazardous contaminants, precision medicine, emerging technologies, and bioinformatics.

SRP Health Scientist Administrator Danielle Carlin, Ph.D., gave an invited talk focused on using atherosclerosis as a case study to understand the joint action of environmental chemical and nonchemical stressors.

Soil Conference Explores Connection Between Soil Microbes and Human Health

Henry, left, and Foundation for Food and Agriculture Research Scientific Program Director LaKisha Odom, Ph.D., pictured at the meeting. Odom was a member of the conference planning committee and chaired the session on funding opportunities and challenges.
(Photo courtesy of Heather Henry)

Nearly 200 scientists and organization leaders from diverse backgrounds came together at the recent Conference on Connections Between Soil Health and Human Health, held October 16 – 17 in Silver Spring, Maryland. Coordinated by the Soil Health Institute, the working meeting aimed to provide a roadmap for exploring the connections between soil microbe ecosystems, soil health, food production, nutrition, and human health.

Superfund Research Program (SRP) Health Scientist Administrator Heather Henry, Ph.D., participated in a panel discussion about funding opportunities for research linking soil health and human health.

"SRP's transdisciplinary research approaches serve as an excellent model for addressing the complex research questions identified at the meeting," noted Henry. "Several of our grantees are leaders in understanding the connections between human health as a function of soil-plant-contaminant interactions – both in terms of research and community engagement."

Henry described several relevant SRP-funded research projects, including studies about mechanisms to limit arsenic uptake in rice, minimizing contaminant uptake in urban gardens, understanding exposure pathways through contaminated soil, and the impact of biota (plants, fungi, or bacteria, for example) on reducing contamination.

Other attendees included representatives from the U.S. Food and Drug Administration and scientists with expertise in soil health, food quality and food security, human health, bioavailability, land management, toxicology, and soil cleanup. The workshop leveraged the multidisciplinary expertise of participants to develop ten recommended research priorities. These priorities include understanding how microbiome structure and function relate to human and soil health; integrating existing human health and soil health data; and enhancing communication strategies between health, agricultural, and environmental sectors.

One Year Later, SRP Researchers Investigate the Health Impacts of Hurricane Maria

PROTECT team members delivered water filtration systems to health centers so community members would have a source of drinkable water.
(Photo courtesy of the Northeastern SRP Center)

After Hurricane Maria made landfall in Puerto Rico as a Category 4 storm in September 2017, Northeastern University Superfund Research Program (SRP) researchers immediately responded, reaching out to participants involved in an ongoing study on exposure impacts in pregnant women and providing essential supplies. Now, more than one year later, they continue to investigate the effects of Maria, including the potential long-term health impacts from exposure to contaminated water and air.

The Northeastern SRP Center, the Puerto Rico Testsite for Exploring Contamination Threats (PROTECT), began recruiting pregnant women in 2010 to assess the relationship between preterm birth and exposure to potentially hazardous contaminants in Puerto Rico. In the months following the devastation from Maria, PROTECT team members provided supplies, including diapers, mosquito nets, and water filters, and developed educational materials related to potential health impacts of water use. They also continued collecting groundwater and tap water samples, as well as hair and blood samples from study participants.

PROTECT researcher Ingrid Padilla, Ph.D., is comparing tap and groundwater samples collected after the hurricane with previously collected data to see what effects the hurricane may have had on contamination in these water systems. As she explained to Chemical & Engineering News, flooding of Superfund sites may have contributed to a spread of pollutants into water systems, such as trichloroethylene and other chlorinated volatile organic chemicals, and these pollutants have been linked to effects on the liver, kidneys, and immune and reproductive systems. Her team is currently comparing samples they took a month after the storm with those before the storm to see whether the concentrations of some chemicals have increased.

PROTECT researchers led by Akram Alshawabkeh, Ph.D., and April Gu, Ph.D., also are investigating Hurricane Maria’s impact on the environment. Alshawabkeh and Gu, along with Northeastern professor Ameet Pinto, Ph.D., received a National Science Foundation Rapid Response Research grant, Timely Assessment of Water Quality to Reveal the Potential Ecological and Health Impact of Hurricanes at Puerto Rico. Using state-of-the-art toxicity tests, the team is assessing the effects of Hurricanes Irma and Maria on Puerto Rico’s sewage system, drinking water supply, and surface water.

Simplifying PCB Concentration Calculations in Sediment

PCB passive sampling frame embedded in sediment. Passive samplers are devices designed to sample the surrounding environment without pumping. (Sanders JP, Andrade NA, Ghosh U. 2018. Evaluation of passive sampling polymers and nonequilibrium adjustment methods in a multiyear surveillance of sediment porewater PCBs. Environ Toxicol Chem 37(9):2487-2495.)

Simple adjustment methods perform as well as or better than complex methods for calculating porewater concentrations of polychlorinated biphenyls (PCBs), according to new research from SRP-funded engineering professor Upal Ghosh, Ph.D. Porewater is the water that fills the spaces between particles of sediment.

Ghosh, a researcher at the University of Maryland, Baltimore County, and his team used polymer passive samplers to measure how well amendments, which bind to contaminants in the environment, remove PCBs from porewater in a contaminated marsh. They measured the concentrations of PCBs in porewater for two years after the sediments were treated with amendments and compared the findings to porewater in sediments not treated with amendments. They also used several methods to fine-tune these measurements to determine which approach performed best.

Researchers commonly adjust passive sampler measurements to take into account conditions that may prevent contaminants from flowing equally through passive samplers and the surrounding environment, but some approaches are more difficult and complicated than others. In comparing the performance of these calculations, the team generally found that the different methods produced similar estimated porewater PCB concentrations.

Because the simple methods provided similar results to the more complex methods, the authors suggest that they can provide reasonably accurate concentration adjustments in scenarios when contaminants do not flow equally through the samplers and the environment. In cases in which the sediments were amended with either activated carbon or SediMite, a novel remediation technology patented by Ghosh, the simple approach performed better than the more complex adjustment method.

SediMite is an amendment made of activated carbon, clay, and sand that is engineered into pellets for easy handling and delivery into the water column. The pellets are designed to slowly disperse and mix in the sediment to bind to contaminants and reduce their uptake in the aquatic food chain.

EPA Adds Five Hazardous Waste Sites to the National Priorities List

EPA National Priorities List (NPL) sites. Current NPL sites are shown in yellow, proposed sites are shown in red, and deleted sites are shown in green. See the full map for more information.

The U.S. Environmental Protection Agency (EPA) recently announced that it added five hazardous waste sites to the National Priorities List (NPL) of Superfund sites. An additional six proposed listings are currently open to public comment. A total of 1,345 uncontrolled hazardous waste sites are currently listed.

The added sites in Indiana, Mississippi, Ohio, Tennessee, and Texas include former automotive and munitions manufacturing sites, groundwater plumes, and others.

Superfund is the federal program that investigates and cleans up complex, uncontrolled, or abandoned hazardous waste sites in the country. Proposed and newly added NPL sites may offer opportunities for SRP grantees to conduct research.

Suk Receives Fulbright Award to Support Environmental Health in Thailand

Suk is the founding director of the SRP and has served in this role since its inception in 1987.

Superfund Research Program (SRP) Director William A. Suk, Ph.D., M.P.H., has been selected to receive a prestigious Fulbright U.S. Scholar Program award to increase environmental health capacity in South and Southeast Asia. As part of the international educational exchange program, Suk will serve as Fulbright lecturer in international and public health in collaboration with Mahidol University and the Chulabhorn Research Institute in Bangkok, Thailand.

During his six-month Fulbright, Suk will teach courses and aid in program development for universities in Thailand and surrounding countries, with an emphasis on children’s environmental health and early-life exposures that lead to disease. He also will address global health concerns, such as chronic kidney disease of unknown origin (CKDu) and potentially harmful exposures from electronic waste, and will promote the development of tools and technologies to reduce exposures.

Suk will meet with researchers, postdocs, students, pediatricians, environmental health scientists, public health workers, and governmental ministries in South and Southeast Asia to discuss how environmental exposures may lead to disease and the importance of developing ways to intervene and prevent these diseases. He also will promote the SRP as a model for other universities to develop similar multidisciplinary research and training programs.

The Fulbright Program is sponsored by the U.S. government and is designed to build lasting connections between the people of the U.S. and those of other countries. Recipients of Fulbright awards are selected based on their academic and professional achievements, as well as record of service and demonstrated leadership in their respective fields.

Method Predicts Bioavailability of Mercury in Water and Sediment

The researchers found that the amount of mercury taken up by the DGT passive sampler was a good indicator of mercury bioavailability.
(Photo courtesy of Environ Sci Technol, Ndu et al., 2018)

In a new Superfund Research Program (SRP) study, researchers show how a simple sampler can be used to estimate mercury bioavailability – the amount of mercury that can be taken up by organisms – in sediment and water.

In mercury-contaminated sediment and water, bacteria can convert mercury into methylmercury, its more toxic and more bioavailable form. Because of this process, the bioavailability of mercury to these microbes and the production of methylmercury by bacteria are critical steps toward bioaccumulation of methylmercury in fish.

To better predict mercury bioavailability at a contaminated site, researchers led by Duke University scientist Heileen Hsu-Kim, Ph.D., investigated how different types of samplers can be used to measure the amount of mercury that is bioavailable in the environment. They compared several detection methods and found that sampling devices placed in water or sediment to detect a variety of metals, called diffusive gradient in thin-film passive samplers (DGT passive samplers), were the best predictor of mercury bioavailability. They found that the net production of methylmercury correlated well with the accumulation of mercury on the DGT passive samplers.

The study was supported by an SRP individual project grant focused on evaluating mercury methylation potential during remediation of contaminated sediments. According to the authors, this method has the potential to help researchers and site managers improve their understanding of the factors controlling mercury methylation at field sites. This could help them to predict how changes at the site, such as remediation efforts, might alter methylmercury production.

Dartmouth Superfund Research Program Informs International Mercury Reduction Efforts

Chen is studying the cycling of mercury (Hg2+) and its more toxic form, methylmercury (MeHg), in aquatic food webs.
(Photo courtesy of the Dartmouth SRP Center)

A recent article highlights key research advances and needs to inform international policy decision making related to mercury. The article, co-authored by Celia Chen, Ph.D., of the Dartmouth College Superfund Research Program (SRP) Center, emphasizes the importance of bringing together scientific information to better understand the sources of mercury, its movement through the environment, and its effects on human and ecosystem health. Chen is an internationally recognized researcher on the accumulation of metals like mercury in aquatic food webs and serves as director of the Dartmouth SRP’s Research Translation Core.

Mercury contamination is a global problem. Recent advances in the measurement of mercury have helped scientists identify and monitor mercury in the environment. They have found that mercury levels in the atmosphere have been going down in North America and Europe; however, these levels have been increasing in East Asia and the Southern Hemisphere. More research is needed to understand mercury use and transport at the global scale.

Chen addressed the Conference of the Parties (COP) signatories to the Minamata Convention on Mercury in September 2017. The next COP will be held in November 2018.
(Photo courtesy of Celia Chen)

This understanding is important as mercury can have adverse effects in humans, such as nervous system and digestive system toxicity. Symptoms can include memory loss, tremors, insomnia, and cognitive and motor dysfunction. Humans can be exposed to mercury through eating fish that contain a toxic form of mercury called methylmercury. Chen and other researchers are learning how mercury in the environment becomes available to organisms like fish and transforms into methylmercury. They also are developing effective ways to educate communities that rely on fish for their diet.

More research is also needed to understand how climate change might influence mercury movement through the environment. For example, during wildfires, mercury that was absorbed into plants can be released into the environment again. With predictions of more wildfires and other factors related to climate change, scientists are researching these processes to help policymakers with science-based decision making and land-use planning.

The recent paper is based on important findings and next steps discussed at the SRP-sponsored 13th International Conference on Mercury as a Global Pollutant (ICMGP-13), held in Providence, Rhode Island, in July 2017. A major goal of the conference was to inform policy changes in support of the Minamata Convention, which aims to protect human health and the environment from mercury releases. ICMGP-13 organizers previously commissioned four papers, published in the journal Ambio, to communicate important information from the conference.

SRP Well-Represented at National ISES-ISEE Meeting

Vieira discusses her research while networking during one of the session breaks.
(Photo courtesy of Joey Rudd)

Superfund Research Program (SRP) grantees from all over the country gathered in Ottawa, Canada August 26 – 30 for the joint annual meeting of the International Society of Exposure Science and the International Society for Environmental Epidemiology (ISES-ISEE). Current and former SRP grantees and trainees presented their innovative research in epidemiology and exposure science during oral and poster sessions.

Veronica Vieira, Sc.D., an associate professor at the University of California, Irvine, and former Boston University SRP project leader, co-chaired and organized the meeting. She also gave a talk about the relationship between chemical and non-chemical exposures and risk-taking behavior among adolescents living near a Superfund site.

Trottier discussed how people are exposed to toxic chemicals in electronic waste.
(Photo courtesy of Brittany Trottier)

SRP grantees were prominent in a variety of scientific sessions, including those related to gene-environment interactions, polychlorinated biphenyls (PCBs), endocrine disrupting chemicals, children’s health, and more. Some of the many SRP grantee activities and presentations are described below.

Heather Stapleton, Ph.D., from Duke University, and John Meeker, Ph.D., from the University of Michigan and Northeastern University SRP Center presented during a session on children’s exposure to volatile organic compounds. University of California, Berkeley researcher Rachel Morello-Frosch, Ph.D., discussed exposure to per- and polyfluoroalkyl substances and biomarkers of stress response during pregnancy.

Kathleen Vandiver, Ph.D., from Massachusetts Institute of Technology, and Esther Erdei, Ph.D., of the University of New Mexico chaired a session focused on contaminants near indigenous communities, including potential exposures, health effects, and avenues for tribal research. Erdei spoke about research to address the Cheyenne River Sioux Tribe's concerns about mine waste, and Marcella Thompson, Ph.D., assistant professor at the University of Rhode Island and co-leader of the Brown University SRP Center Community Engagement Core, spoke about community engaged research with the Narragansett Tribe during the session.

SRP-funded small business Entanglement Technologies had a booth with information about their chemical vapor analyzer, and how it has been used in emergency response efforts.
(Photo courtesy of Ginger Guidry)

Laurel Schaider, Ph.D., of the Silent Spring Institute and the University of Rhode Island SRP Center, discussed drinking water quality and environmental justice in the United States. Matt Cave, M.D., from the University of Louisville, discussed his work related to the health effects of polychlorinated biphenyls. Columbia University researcher Stephen Chillrud, Ph.D., shared his work related to monitoring air pollutants.

ISES-ISEE included a session on electronic waste chaired by SRP Health Scientist Administrator Michelle Heacock, Ph.D., and Health Specialist Brittany Trottier. It also included a special session on the epidemic of chronic kidney disease of unknown origin, co-chaired by NIEHS Health Scientist Administrator Bonnie Joubert, Ph.D., and SRP grantee Madeleine Scammell, Ph.D., from Boston University. The session built off a June 2018 workshop on kidney disease, and earlier workshops supported by the SRP.

Michigan State University SRP Center Founding Director Lawrence Fischer Dies

Lawrence Fischer, Ph.D.

Lawrence Fischer, Ph.D., former Michigan State University (MSU) professor and founding director of the MSU Superfund Research Program (SRP) Center, died on Tuesday, August 7, 2018. He was 80.

Fischer became the director of the MSU Institute of Environmental Toxicology in 1985. There he brought together researchers with diverse scientific backgrounds to form the MSU SRP Center in 1988, which is presently the longest standing program grant at MSU.

"He will be remembered for his open and relaxed demeanor, which was a hallmark of his effectiveness in building campus-wide relationships," said current MSU SRP Center Director Norbert Kaminski, Ph.D. "During his nineteen-year tenure at MSU and under his leadership, our toxicology research and graduate training became nationally and internationally recognized for excellence.

Fischer was instrumental in shaping undergraduate, graduate, post-graduate, and professional student training in toxicology at MSU. In addition to teaching and training numerous doctoral and postdoctoral students in his own laboratory, he served as the director of the graduate training program in environmental toxicology for 15 years.

Fischer also served on many scientific advisory committees throughout his career, including the Governor's Michigan Environmental Science Board, the Michigan Air Toxics Policy Committee, the U.S. Environmental Protection Agency's Science Advisory Board on Mercury, the Science Advisory Group on Electromagnetic Fields and Childhood Leukemia Study for the National Cancer Institute, and the National Academy of Science Committee on Drinking Water Contaminants.

"Larry was a brilliant scientist and a driving force behind the SRP Center at MSU," said NIEHS SRP Director William Suk, Ph.D. "He was an amazing human being who had a profound impact on his colleagues, students, friends, and family."

SRP Visits University of New Mexico METALS Superfund Research Program Center

The UNM Metals External Advisory Board and SRP staff meet with community members at the Red Water Pond Community Association.
(Photo courtesy of Chris Shuey)

Danielle Carlin, Ph.D., and Brittany Trottier of the NIEHS’s Superfund Research Program (SRP) learned how the University of New Mexico Metal Exposure and Toxicity Assessment on Tribal Lands in the Southwest (UNM METALS) SRP Center is working to improve the health of Native Americans in the southwestern United States who are living near abandoned uranium mine waste.

During a site visit on August 7 – 10, 2018, Carlin and Trottier joined UNM METALS SRP Center director Johnnye Lewis, Ph.D., co-director Matthew Campen, Ph.D., and members of the UNM METALS External Advisory Board to visit tribal communities living near abandoned mine sites. Researchers at the sites are studying mobility of metals in soils and particulates like dust and developing new methods to immobilize metals to reduce human exposures. They also are developing new methods to predict how contaminated dust might travel under different weather conditions so tribal communities can protect themselves from exposure.

Lewis and some External Advisory Board members visited the area near the Jackpile mine site where UNM METALS researchers are doing some of their work.
(Photo courtesy of Brittany Trottier)

During their visit, Carlin, Trottier, Lewis, and other Board members attended community meetings at Red Water Pond Road and the Laguna Pueblo. The Laguna Pueblo community is near an abandoned 8,000+ acre site that encompasses the 3,000-acre open pit Jackpile Mine Superfund site. Drawing on multi-directional communication and partnerships with tribal members and federal agencies, the UNM METALS Community Engagement and Research Translation Cores are implementing new approaches to risk communication and education for risk avoidance that integrate indigenous learning models.

The tribal/university partnership was initiated after members of the Navajo Nation expressed concerns about the high frequency of kidney disease in their community, including cases of teens requiring dialysis. The partnership has grown to include studies of other mine-related concerns. For example, UNM METALS SRP Center researchers are looking closely at how metals affect the immune system and whether zinc supplements reduce immunotoxicity.

A video interview with Lewis and a video of one of her conference presentations provide more information about UNM METALS SRP Center research and community partnerships.

SRP Trainee Explores Intersection of Art and Science

Pieces submitted to "The Art of a Scientist" by a Duke University graduate student, and a local artist. The top image is an electrocardiogram from the scientists research, and the bottom is an artistic representation.
(Photo courtesy of Casey Lindberg and Ariana Eily / Duke University Rubenstein Arts Center)

Duke University Superfund Research Program (SRP) Center trainee Casey Lindberg helped organized an exhibit highlighting the intersection of science and art called "The Art of a Scientist." Featuring visuals of lab work by 22 scientists, including photographs, micrographs, botanical prints, watercolors, and videos, the exhibit provided an opportunity for the public to explore scientific concepts in an accessible and engaging format.

In addition to featuring images from their lab work, some scientists created artistic representations of their work, and others were paired with local artists to bring their findings to life.

In an interview with WUNC radio, Lindberg told Frank Stasio that the exhibit was a wonderful way to see science and art intersect seamlessly.

Lindberg, who works in the lab of Richard Di Giulio, Ph.D., is exploring how exposure to polycyclic aromatic hydrocarbons and other stressors impact fish.

She shared information about her research and the exhibit at a recent Duke SRP Center Community Advisory Board meeting. SRP Health Scientist Administrator Heather Henry, Ph.D., and Health Specialist Brittany Trottier attended the meeting and enjoyed hearing about the science and art exhibit, as well as other efforts by the Duke SRP Center to engage local communities.

Duke University SRP Center Community Engagement Core Community Advisory Board Meeting participants.
(Photo courtesy of Brittany Trottier)

Early SRP Collaboration Guides Potential Treatment Options for Disorders of the Eye

Hammock, left, and Hennig, right, began collaborating nearly 20 years ago to understand the underlying mechanisms of disease and to identify potential targets for treatment.
(Photo courtesy of the University of California, Davis and the University of Kentucky, respectively)

Researchers are one step closer to determining how to prevent blindness from occurring in mice with macular degeneration and diabetic retinopathy, two diseases that affect the eye. This work stems from a collaboration dating back nearly 20 years between SRP-funded researchers Bruce Hammock, Ph.D., from the University of California, Davis, and Bernhard Hennig, Ph.D., from the University of Kentucky.

Their early research showed that exposure to polychlorinated biphenyls damaged the protective barrier of cells that line blood vessels in the body. This damage can lead to oxidative stress, inflammation, and eventually diseases like atherosclerosis, when substances build up on the artery’s walls. They also found that breakdown products of certain dietary fats made this process worse. An enzyme called soluble epoxide hydrolase (sEH), originally discovered in Hammock’s lab, is responsible for breaking down fats in the body after they are metabolized.

The collaborators showed that they could prevent harmful breakdown products from forming and protect the cells lining blood vessels in the body by inhibiting sEH. Hammock and colleagues also recently reported that inhibiting sEH protected mice prone to macular degeneration from developing abnormal blood vessels in the eye, which are responsible for vision loss.

Building on these findings, a new paper led by a collaborator of Hammock, Ingrid Fleming, Ph.D., showed that sEH sets off a similar chain of events to produce harmful breakdown products that damage the blood vessels of the eye, leading to diabetic retinopathy, a condition in which the blood vessels of the eye become damaged, and can lead to blindness. Published in the journal Nature, their findings showed that inhibiting sEH prevented mice from developing the disease, and they suggest that sEH inhibitors may offer new treatment options.

“The findings of this paper and the earlier work it builds off of exemplify the uniqueness of the SRP,” said Hennig. “Their emphasis on multidisciplinary research provides opportunities that lead to major scientific discoveries.”

Global Pollution Observatory Provides Path to Addressing Global Pollution

Landrigan discussed the Lancet Commission report at the GBD Workshop in March 2018.
(Photo courtesy of Howard Hu)

Following a workshop and commentary, environmental health experts are one step closer to the formation of a Global Pollution Observatory (GPO), a major recommendation of the Lancet Commission report on pollution and health to create a multinational consortium to assist countries in prioritizing pollution initiatives. The GPO is intended to help researchers in diverse disciplines collect and analyze data to inform policy and intervention approaches to reduce the global burden of disease (GBD).

In a workshop at the University of Washington, GBD Project members and environmental health experts discussed how to leverage the Commission report and create a strategic framework for the GBD Pollution and Health Initiative, including the formation of the GPO.

Building off the Commission report and the efforts of the workshop, a new commentary in the journal Environmental Health Perspectives describes how the GPO will provide a path to controlling global pollution and protecting health. Authored by leaders in the field, including former SRP grantees Philip Landrigan, M.D., and Howard Hu, M.D., as well as SRP Director Bill Suk, Ph.D., the commentary explains how the GPO will compile, geocode, store, and analyze data to discern trends and geographic patterns. According to the authors, this resource will provide critical insight for policy makers, the media, and the public. It will also enable researchers to track pollution and pollution-related disease around the world, track progress in disease reduction over time, and generate new research questions.

Focusing on the preventable nature of pollution, the authors also stress how many high- and upper-middle-income countries have developed useful and cost-effective pollution control technologies, many of which are ready to be brought to the global scale to address the GBD in middle- and low-income countries.

“The recent commentary shows the momentum and the actions emerging as a result of the Lancet Commission report,” said Suk. “The Global Pollution Observatory provides a concrete path forward towards addressing pollution and protecting human health.”

CycloPure Chemist Wins Guggenheim Fellowship

Dichtel is also a professor of chemistry at Northwestern University.
(Photo courtesy of the MacArthur Foundation)

William Dichtel, Ph.D., co-founder of the NIEHS-funded small business CycloPure, was recently awarded a prestigious 2018 Guggenheim Fellowship for his ongoing work in polymer chemistry. CycloPure's NIEHS grant, administered by the Superfund Research Program, focuses on developing new high-affinity polymers derived from corn to remove per- and polyfluoroalkyl substances (PFASs) from water.

PFASs are a group of chemicals that have been widely used in manufacturing, food packaging, firefighting materials, and commercial household products, such as nonstick products, polishes, and cleaners. Exposure to PFASs, which have been found in drinking water systems, has been linked to weakened immunity, thyroid disease, and cancer.

Currently, activated carbon is the most widely used PFAS remediation technology. Limitations of this approach include susceptibility to fouling by organic matter, energy-consuming regeneration processes, and low affinities for PFASs, resulting in requirements for substantial amounts of materials and energy.

CycloPure's adsorbent technology is based on a family of molecules called cyclodextrins, which are easily made from cornstarch at low cost and form pockets that can efficiently adsorb contaminants. The new technology organizes these naturally "sticky" cyclodextrins into a porous, crosslinked material that captures thousands of contaminants, including PFASs. With its NIEHS grant, Cyclopure is refining its cyclodextrin polymerization process to prepare for scaling up production for commercial use.

Dichtel's breakthroughs in polymerization and material sciences also brought him recognition as one of the top-ten national finalists for the Blavatnik Award for Young Scientists in 2017 and won him a Genius Grant from the MacArthur Foundation in 2015. He was highlighted as one of the "40 Under 40," an innovator who "puts an outsize mark" on Chicago business and nonprofits, by Crain's Chicago Business in 2017. To learn more, check out a video on his polymer research on the Big Ten Network.

SRP Startup Nets Innovative Technology Prize

Lippert, right, receives the China BlueTech Most Innovative Technology Award.
(Photo courtesy of the China BlueTech Awards)

At the 2018 China BlueTech Awards, the Most Innovative Technology Award went to SRP-funded small business PowerTech Water for its next-generation water purification technology, called INCION.

The BlueTech Awards offer a platform for innovative water and wastewater companies to demonstrate readiness for the Chinese market and to network with industry experts, corporations, and investors.

INCION uses an active filtration process with proprietary carbon electrodes to eliminate metals from water under the influence of small voltages. The system is highly specific for metals, such as lead, iron, chromium, and manganese, and provides a longer device lifetime than competing technologies. In addition, INCION can remove chlorine, volatile organic contaminants, taste, and odor from drinking water.

PowerTech Water co-founder and CEO Cameron Lippert, Ph.D., accepted the award in Shanghai, China. “I would like to thank the judges for recognizing our hard work and determination to succeed in the China market, which we view as an exciting opportunity for us going forward,” Lippert noted.

International Conference Promotes Environmental Health Research Exchange

The conference brought nearly 200 scientists from 18 countries together to engage in discussions addressing health and environmental issues.
(Photo courtesy of the LSU SRP Center)

NIEHS Superfund Research Program (SRP) staff and grantees focused on environmental health issues in changing economies at the Central and Eastern European Conference on Health and the Environment (CEECHE), June 10 – 14 in Krakow, Poland. The conference, sponsored in part by the SRP, provided a forum for scientists, engineers, and organizations to focus on Central and Eastern European environmental health concerns, which also will inform practices around the world. Presentations, including several by SRP grantees, described the health effects of exposure to environmental chemicals and highlighted promising approaches to prevent or reduce pollutants in the region. Sessions highlighted health risks and solutions for atmospheric air pollution, environmental issues at former military sites in Central and Eastern Europe, coastal and aquifer pollution, health impacts of mining activities, and other emerging environmental health topics.

SRP Centers at Louisiana State University and the University of Kentucky collaborated to organize the conference.

"CEECHE integrates scientific disciplines to help us solve environmental health issues in this region and elsewhere," said SRP Director Bill Suk, Ph.D. During his plenary talk, Suk discussed emerging and recurring environmental health issues and the importance of advancing science as environmental concerns emerge.

Former Oregon State University SRP Center trainee Erin Madeen, Ph.D., left, discusses her research at the CEECHE poster session. Madeen also organized several training activities, including a career panel featuring academic, government, and entrepreneurship career pathways in both Europe and the U.S.
(Photo courtesy of Heather Henry)

During the meeting, scientists presented data linking exposures to various environmental chemicals with significant health impacts, including infertility, birth defects, cardiovascular disease, and increased mortality associated with infections. Other scientists presented innovative ways to combat pollution. For example, scientists described how effective planting and management of trees that can accumulate heavy metals from soil may improve urban green areas and reduce pollution.

SRP Health Scientist Administrator Heather Henry, Ph.D., gave an overview of SRP grantees who are developing innovative tools and methods for coastal and aquifer remediation and monitoring.

Slawomir Lomnicki, Ph.D., from the Louisiana State University SRP Center, served as a CEECHE 2018 conference co-chair, along with Stanisław Gawronski, Ph.D., of the Warsaw University of Life Sciences. Together, they worked closely with conference program committee co-chairs Kelly Pennell, Ph.D., assistant director of the University of Kentucky SRP, and Florian Gambus, from the University of Agriculture in Krakow.

Ferguson Presents Tools to Assess Unknown Environmental Chemicals

Henry introduced Ferguson and moderated a lively Q&A session that followed.
(Photo courtesy of Steven R. McCaw, NIEHS Multimedia Services)

At the July 10 Keystone Science Lecture, Duke University SRP Center researcher Lee Ferguson, Ph.D., discussed his work using non-targeted approaches to identify contaminants in water, dust, and other environmental media. His talk drew a wide audience from across the Institute.

Ferguson explained how his team is using tools originally developed to study genes and proteins and applying them to study chemicals in the environment. He described how the traditional approach, called targeted analysis, looks for specific compounds in environmental samples. In contrast, Ferguson's group looks at everything detected in an environmental sample and then tries to identify the compounds. This approach is called untargeted analysis.

"There are 60 to 120 million unknown chemical compounds out there," noted Ferguson. "By combining high-resolution mass spectrometry, chemical databases, and computational approaches, we can assign molecular and structural formulas to unknown compounds."

Ferguson explained how scientists use different lines of evidence to identify unknown compounds in environmental samples.
(Photo courtesy of Steven R. McCaw, NIEHS Multimedia Services)

Ferguson described several projects where this approach helped identify contaminants that people may be exposed to in water, soil, or dust. In one example, the team found approximately 55,000 unique compounds in water samples from a variety of sources. They were able to tentatively identify about 300, including agricultural chemicals, pesticides, flame retardants, pharmaceuticals, and plastic additives. Nine of the compounds previously had not been reported as environmental contaminants.

Ferguson's team determined that some of the contaminants had persisted through the wastewater treatment process to end up in rivers. Others were measured in drinking water and, according to Ferguson, likely were introduced through building infrastructure such as pipes.

"Dr. Ferguson's research shows that while there are many compounds people can be exposed to, only a small fraction is easily identified," said SRP Health Scientist Administrator Heather Henry, Ph.D. "Their approaches help to sift through large amounts of data to identify compounds that might be harmful to humans or the environment. This research supports the concept of the exposome, or all the exposures a person experiences over the course of life, which is an important part of the NIEHS Strategic Plan."

SRP Grantees Discuss Kidney Disease at NIH Workshop

Navas-Acien discussed her work linking metals exposure to kidney disease.
(Photo courtesy of Brittany Trottier)

Clinicians, basic scientists, epidemiologists, and public health officials met June 25 – 26 to develop a coordinated research agenda for a growing epidemic of chronic kidney diseases. The workshop, held in Bethesda, Maryland, was jointly sponsored by NIEHS and the National Institute of Diabetes and Digestive and Kidney Diseases.

Participants discussed current gaps in knowledge and a path forward to better understand the causes of – and potential treatments for – chronic kidney diseases in agricultural communities. SRP Director Bill Suk, Ph.D., and SRP Health Specialist Brittany Trottier were members of the organizing committee and attended the meeting, largely organized by Bonnie Joubert, Ph.D., of NIEHS.

Presenters included SRP grantees Ana Navas-Acien, Ph.D., from Columbia University, and Madeleine Scammell, D.Sc., from Boston University. Navas-Acien described the link between exposure to metals, such as cadmium, lead, and arsenic, and chronic kidney disease. Scammell discussed a unique epidemic of chronic kidney disease that disproportionately affects young male agricultural workers in El Salvador. She explained how her team has been working closely with the impacted community and the importance of building trust.

"This workshop gathered experts from across disciplines to discuss a complex environmental health problem and how to address it," said Suk. "SRP has supported a number of researchers examining the connection between environmental exposures and kidney disease, and funded early workshops in Costa Rica and Sri Lanka that really put kidney disease on the NIEHS radar."

PCTech Graduates from Competitive Commercialization Program

Krutkramelis leads the SRP-funded small business project to develop the X-FA system.
(Photo courtesy of Kaspars Krutkramelis)

In early June, Superfund Research Program small business grantee and Wyoming-based start-up Pollution Control Technologies (PCTech) completed a nine-month NIH Commercialization Accelerator Program (CAP). The competitive program provides NIH's most promising small business grantees with expert mentors to help them establish market and customer relevance, build commercial relationships, and focus on revenue opportunities.

PCTech is in the process of commercializing its X-FA system, a cost-effective tool to capture mercury emissions. The X-FA tool is composed mainly of fly ash, a waste product at power plants that usually requires its own special management. Using an innovative method, PCTech deposits a thin coating of activator material around the recycled fly ash particles to create an X-FA sorbent that can chemically bind to mercury. The X-FA sorbent, which is less expensive than traditional mercury removal technologies, can be produced on-site at power plants and can capture large amounts of mercury.

In a closeout webinar on June 11, PCTech presented its accomplishments during the CAP, as well as its future plans. As part of the program, PCTech created a commercialization strategy toolkit and received individualized mentoring on ways to achieve market readiness.

"NIH CAP benefited us substantially through advising, conference calls, and connections in the industry sector," said PCTech project leader Kaspars Krutkramelis, Ph.D. Krutkramelis and his team are actively beta testing their product with several energy utility companies.

PCTech is now testing the use of its product in commercial air purification filters.
(Photo courtesy of Kaspars Krutkramelis)

"The program also helped us to brainstorm new ideas related to mercury capture that reaches past our current product," Krutkramelis added. Based on suggestions to seek alternative customers and products for the active chemical compound, PCTech has started testing its product in commercial air purification filters. The company also plans to introduce its product to other sectors that use mercury, such as dentistry, where it is used in dental amalgams.

"NIEHS encourages small business grantees to take advantage of NIH's CAP program," said SRP Health Scientist Administrator Heather Henry, Ph.D. "Participation requires an investment of time; however, we've noticed that small businesses make real breakthroughs as part of the CAP process, resulting in an expanded customer base. PCTech's exploration of the commercial air filter market is a great example of this."

SRP Grantees Share Findings and Resources with Stakeholders

Members of the Dartmouth College Superfund Research Program (SRP) Center have actively engaged community members, regulatory partners, and lawmakers by sharing research results and resources they can use. In keeping with the SRP's strong emphasis on community engagement and research translation, the Dartmouth SRP Center has taken steps to ensure its research findings are shared with stakeholders in a useful and informative manner.

Arsenic in Food and Water

Arsenic and You website
(Photo courtesy of the Dartmouth SRP Center)

Dartmouth SRP Center members have developed materials to explain how people are exposed to arsenic and how they can prevent or reduce their exposures.

The team provided fact sheets and magnets promoting their Arsenic and You website to partners at the New Hampshire Department of Environmental Services (NHDES) and Department of Health and Human Services (NH DHHS). The materials were used in a community presentation aimed at private well users to help answer questions about arsenic in food, water, and other sources.

NHDES and NH DHHS cited Dartmouth SRP Center research in a series of press releases coinciding with National Drinking Water Week, beginning May 7. One press release stressed the importance of testing wells for arsenic, quoting Center researcher Margaret Karagas, Ph.D., and her team's work to understand the health impacts of exposure to low levels of arsenic.

The team also provided information to a U.S. Representative's office about arsenic found in food and rice. The information was related to a set of five publications out of the Dartmouth SRP Center about the sources and pathways of arsenic in food.

Addressing Mercury Contamination

Dartmouth SRP Center researchers have explored how environmental factors, such as temperature, salinity, and microbial activity, influence the movement of mercury from sediment and water into the food web and ultimately to humans.

Project leader Celia Chen, Ph.D., shared her experience working with collaborators across the globe to produce four papers for a special issue of the journal Ambio describing the latest science about mercury. As part of an invited talk at Plymouth State University, she used this work to explain how science can help inform environmental policy. The papers will inform activities under the Minamata Convention, a global treaty on mercury that was ratified in 2017.

Center researchers also have formed partnerships to help reduce exposure to mercury. For example, several researchers, core leaders, and coordinators from the Dartmouth SRP Center met with partners from the U.S. Environmental Protection Agency who manage remediation at two Superfund sites. They provided updates on their research related to mercury and discussed how Center activities could better support cleanup efforts at the sites.

Sunderland Discusses PFAS Research at ATSDR

Sunderland is an associate professor of environmental science and engineering at the Harvard School of Public Health. She leads a project for the University of Rhode Island SRP Center.
(Photo courtesy of the Harvard School of Public Health)

At a May 10 seminar, Elsie Sunderland, Ph.D., described her research efforts to understand how people are exposed to poly- and perfluoroalkyl substances (PFASs) and to develop quantitative tools tracing PFAS exposures back to their sources. PFASs are a large group of manufactured compounds that have been widely used since the 1950s to make everyday products more resistant to stains, grease, and water. In animal studies, some PFASs have been shown to disrupt endocrine activity, reduce immune function, and bring about developmental problems.

Sunderland's talk at the Agency for Toxic Substances and Disease Registry (ATSDR) in Atlanta was part of the SRP-ATSDR Seminar Series. Hosted by the NIEHS Superfund Research Program (SRP), ATSDR, and the National Center for Environmental Health, the seminar was followed by a lunch discussion and afternoon meetings with staff from ATSDR and other local partners. The SRP-ATSDR seminars highlight the most recent SRP research results relevant to ATSDR and help SRP scientists identify potential future research needs.

Sunderland, an associate professor at the Harvard School of Public Health and project leader at the University of Rhode Island SRP Center, described how her team uses novel statistical methods to link PFASs measured in surface waters and fish to their sources. She also explained how they are using these methods to analyze human serum data to understand which sources of PFASs contributed most to human exposures. They have found that while seafood was an important source for some types of PFASs in certain populations, consumer products were the largest source of PFASs overall.

Successful SRP Webinar Series Focuses on Toxicity Testing

In the spring 2018 Risk e-Learning webinar series, Superfund Research Program (SRP) grantees and colleagues featured research and technologies that may be useful for evaluating the safety of chemicals. These approaches aim to replace or reduce the use of animal models, test more chemicals in a shorter period of time, and generate findings that are more relevant to humans. In total, this series attracted 1,022 live participants, 420 online archive views, and 3,128 video podcast downloads.

On the May 14 kickoff session, Toxicity Testing Strategies and Model Systems, presenters provided an overview of toxicity testing strategies to advance the use of 21st-century science in chemical safety evaluation. Speakers also described the pros and cons of model systems, such as zebrafish and cell-based assays, to explore chemical safety. Presenters included Nicole Kleinstreuer, Ph.D., deputy director of the National Toxicology Program's Interagency Center for the Evaluation of Alternative Toxicological Methods; Lisa Truong, Ph.D., a member of the Oregon State University SRP Center; and April Rodd, Ph.D., a postdoctoral researcher at Brown University and a former Brown SRP Center trainee.

The May 23 session, Tools for Assessing Exposure and Toxicity, outlined how toxicity data from one chemical may be used to identify other potential toxicants. Presenters also explored rapid screening tools to assess chemical contaminants in the environment and highlighted genetic screening tools to examine mechanisms of toxicity. Presenters included James W. Rice, Ph.D., a former SRP trainee who now works at Gradient; Erin Baker, Ph.D., a Pacific Northwest National Laboratory researcher and member of the Texas A&M University SRP Center; and Chris Vulpe, M.D., Ph.D., a professor at the University of Florida and a University of California, Berkeley SRP Center collaborator.

The final session on May 31, Modernizing Safety Testing, focused on new and emerging strategies for chemical safety evaluation. The discussion included work to understand how studies using animals, cells, and computer models can address population variability and how in vitro high-throughput assays can provide useful information for assessing the safety of complex mixtures. Presenters included SRP Director Bill Suk, Ph.D.; Weihsueh Chiu, Ph.D., leader of the Texas A&M University SRP Center’s Decision Science Core; and Michael DeVito, Ph.D., acting chief of the National Toxicology Program Laboratory.

More details and archives of each session are available on the SRP Risk e-Learning series page. Risk e-Learning webinars are conducted by the SRP in collaboration with the U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management. The two-hour sessions focus on sharing innovative treatment and site characterization technologies with EPA risk assessors and regional project managers, state and local regulatory agencies, environmental engineering and consulting firms, and academia.

Distinguished Lecture Highlights Mechanisms of Liver Cancer

Following the talk, Karin met with SRP staff to discuss his research. From left, SRP Health Scientist Administrator Heather Henry, Ph.D.; Karin; SRP Health Specialist Brittany Trottier; SRP Health Scientist Administrator Michelle Heacock, Ph.D.; and Suk.
(Photo courtesy of Michelle Heacock)

In a May 15 seminar at NIEHS, Michael Karin, Ph.D., detailed the sequence of molecular changes in the liver that eventually lead to liver cancer. Karin, a Distinguished Professor of Pharmacology and Pathology at the University of California, San Diego (UCSD) School of Medicine, is part of the UCSD Superfund Research Program (SRP) Center.

SRP Director Bill Suk, Ph.D., hosted the talk. In his introduction, Suk explained that Karin has studied the relationship between inflammation, cancer, and metabolic disease at UCSD since 1986.

The Karin group is interested in nonalcoholic steatohepatitis (NASH), one of the more serious illnesses in a group of metabolic disorders known as nonalcoholic fatty liver disease (NAFLD). NAFLD, which affects one-third of U.S. adults, starts as simple fatty liver, but 10–15 percent of patients will develop NASH.

"The factors that control the switch from simple fatty liver to NASH are not clear," Karin said, "but we speculate that it is associated with stress in a part of the cell called the endoplasmic reticulum (ER)."

The ER is a network of tubules in plant and animal cells that produce lipid and proteins. Karin and his colleagues decided to test the hypothesis that the ER was involved using mice that are more likely to develop ER stress in the liver.

In these mice, Karin and his colleagues revealed that a high-fat diet increases ER stress, which activates proteins that increase the production of cholesterol and fatty acids. This process gives rise to cell death and inflammation and, ultimately, the progression from simple fatty liver to NASH.

"Not only does Karin’s work in mice provide a new model for understanding the development of NASH, but it may also uncover what happens in the steps from NASH to liver cancer," said Suk.

Public Webinar Outlines Latest PFAS Exposure and Health Research

The BU SRP Center Research Translation Core has partnered with CHE since 2011 to bring BU SRP Center research into the public spotlight through webinars and calls.

New information on per- and polyfluoroalkyl substances (PFAS) in drinking water and potential health effects was the focus of a May 1 Collaborative on Health and the Environment (CHE) webinar. The webinar was co-organized by the Boston University (BU) Superfund Research Program (SRP) Center.

PFAS chemicals have received increasing attention because they have been found in several drinking water systems and have been linked to reproductive, developmental, liver, kidney, and immunological effects.

In a December 2016 CHE call, presenters outlined the problem of PFAS in drinking water. Because of continued interest in the topic and the high volume of emerging research, organizers held this follow-up webinar to delve into practical information about PFAS testing and interpretation of contamination data, as well as new findings on potential health effects. Speakers included BU SRP Center grantee Tom Webster, D.Sc.; Nancy Rothman, Ph.D., CEO and Principal Scientist of New Environmental Horizon, Inc.; and Richard Spiese, of the Vermont Department of Environmental Conservation, Waste Management Division.

Talks by Rothman and Spiese focused on the latest methods for measuring PFAS in drinking water and how PFAS contamination in drinking water is being addressed.

"The field of expertise is expanding quickly, and there are a lot of resources to help you keep informed on changes to regulations and methods for testing," said Rothman. She emphasized the importance of using labs with experience in PFAS analysis and described the data needed to accurately measure PFAS in community drinking water.

Spiese described lessons learned on installing and operating systems to remove PFAS at impacted residences in Vermont. He discussed system installation and their process for inspection, maintenance, and long-term monitoring of PFAS concentrations.

Webster followed the first two talks with the current state of the science on the health effects of PFAS exposure.

"We have seen an explosion of research on PFAS since the last call in December 2016," Webster said. "Since then, about 40 PFAS epidemiology papers have been published linking PFAS to health outcomes." These studies have reported associations between increased PFAS exposure and higher risk of preterm birth, thyroid hormone disruption, and decreased visual motor ability.

The webinar was moderated by BU SRP Research Translation Core leader Wendy Heiger-Bernays, Ph.D. In her introduction, she provided a short overview of the SRP and encouraged participants to learn more about the University of Rhode Island SRP Center, which is focused on sources, transport, exposure, and effects of PFAS.

EPA Adds Six Hazardous Waste Sites to the National Priorities List

EPA National Priorities List (NPL) sites. Current NPL sites are shown in yellow, proposed sites are shown in red, and deleted sites are shown in green. See the full map for more information.

The U.S. Environmental Protection Agency (EPA) recently announced that it added six hazardous waste sites to the National Priorities List (NPL) of Superfund sites. In addition, EPA is proposing to add four additional sites to the list.

The added sites in Delaware, Indiana, Minnesota, South Carolina, and Texas include former textile manufacturing sites, former metal finishing and electroplating sites, and groundwater plumes.

Superfund is the federal program that investigates and cleans up complex, uncontrolled, or abandoned hazardous waste sites in the country. Proposed and newly added NPL sites may offer opportunities for SRP grantees to conduct research.

SRP Brings Solution-Oriented Science to SOT

Henry, left, with Fry, second from right, and SRP trainees at an SOT poster session.
(Photo courtesy of Heather Henry)

Superfund Research Program (SRP) grantees from all over the country gathered in San Antonio, Texas, for the 2018 Society of Toxicology (SOT) Annual Meeting March 11 – 15. Grantees and staff gave talks and presented posters highlighting SRP-funded research advances in toxicology.

More than 80 SRP project leaders and trainees from at least 13 SRP Centers presented oral or poster presentations. NIEHS SRP staff members Danielle Carlin, Ph.D., Michelle Heacock, Ph.D., Heather Henry, Ph.D., Brittany Trottier, and SRP Director Bill Suk, Ph.D., were on hand to meet with grantees, view their posters, and discuss their innovative research. An SRP reception also gave trainees the opportunity to network.

University of Iowa SRP Center trainee Eric Uwimana describes his research on how polychlorinated biphenyls are metabolized in the body.
(Photo courtesy of Heather Henry)

Some SRP grantees chaired sessions exploring cutting-edge toxicology research. Texas A&M University SRP Center Director Ivan Rusyn, Ph.D., co-chaired a workshop focused on reducing uncertainty when predicting the toxicity of a chemical based on data from similar chemicals. University of North Carolina at Chapel Hill SRP Center Director Rebecca Fry, Ph.D., co-chaired a symposium on the role of epigenetic changes on metal-induced diseases. Epigenetic changes affect the function of genes without changing the underlying DNA sequence.

Carlin also co-chaired a symposium titled, "Atherosclerosis as a Model to Understand the Combined Effects of Environmental Chemical and Non-Chemical Stressors." Presentations covered how chemical and non-chemical stressors may lead to atherosclerosis, biological mechanisms of environmentally relevant chemicals, how diet and physical activity may modify atherosclerotic events, and how conceptual models can be created to evaluate complex pathways in this disease. The session included presentations by University of Kentucky SRP Center Director Bernhard Hennig, Ph.D., and University of Louisville SRP Center Director Sanjay Srivastava, Ph.D.

SRP staff were also available in the NIEHS Research Funding Insights Room, where current grantees and applicants could speak with program officers or scientific review officers about the grants process. In a special session on federal research funding opportunities, Heacock presented an NIH grants overview. Following her presentation, former Boston University SRP Center grantee and current NIEHS Outstanding New Environmental Science awardee Neel Aluru, Ph.D., described keys to success and lessons learned in the NIH grants process.

For more information about NIEHS at SOT, see the Environmental Factor article.

Additional SOT photos of SRP grantees are presented below, courtesy of Heather Henry:

SRP Grantee Presents NIEHS Seminar on Clinical Intervention for Pain

UC Davis SRP Center Director Hammock studies sEH enzyme function and how blocking this function can treat neuropathic pain. His SRP-funded laboratory also pioneered the use of immunoassay technologies to detect hazardous chemicals.
(Photo courtesy of the UC Davis SRP Center)

Renowned scientist and Superfund Research Program (SRP) grantee Bruce Hammock, Ph.D., was invited to present the Immunity, Inflammation, and Disease Laboratory Special Seminar at NIEHS on March 28, 2018. Hammock's talk focused on neuropathic pain, which is caused by damage or disease affecting the body's sensory system, and how findings from his lab are being translated into clinical interventions.

"We currently lack medical tools to address neuropathic pain, and there hasn't been a great deal of interest or ability to come up with new drugs among pharmaceutical companies," Hammock said. "This leaves a critical need for options to treat this pain and improve quality of life for patients suffering from a wide variety of common diseases."

Hammock, who has led the University of California (UC) Davis SRP Center since its inception in 1987, is best known for his discovery of soluble epoxide hydrolase (sEH), an enzyme in cells that degrades chemically stable fatty acid epoxides. He described how blocking the function of this enzyme can reduce endoplasmic reticulum (ER) stress, which is an underlying mechanism by which many diseases produce inflammation and pain.

According to Hammock, blocking sEH function — and therefore ER stress — may be a way to treat neuropathic and inflammatory pain, as well as other diseases stemming from ER stress, such as asthma, fibrosis, diabetic neuropathy, colitis, and depression.

Trainee Research Featured in the SRP Poster Winners Webinar

SRP trainees pictured clockwise from top left: Stephanie Kim, Meichen Wang, Hongyi Wan with Suk, and Kelly Fader.
(Photo courtesy of the trainees)

On March 27, 2018, the four winners from the Superfund Research Program (SRP) Annual Meeting's poster competition presented their outstanding research via webinar to an interdisciplinary audience of SRP staff and grantees.

In his opening remarks, SRP Director Bill Suk, Ph.D., said: "The SRP is a firm supporter of training in the broadest possible way within our multidisciplinary framework. I'm really looking forward to hearing from these four outstanding trainees and for this opportunity for them to share their research with all of you."

The webinar provided a great opportunity for people who were not able to view the student posters at the SRP Annual Meeting to hear the winners describe their current research and future directions:

• Stephanie Kim of Boston University described her use of digital gene expression profiling to understand how metabolism-disrupting chemicals alter the characteristics of fat cells.
• The University of Kentucky's Hongyi (Derek) Wan shared how he has developed membrane platforms with iron and palladium nanoparticles to remove polychlorinated biphenyls from water.
• Kelly Fader of Michigan State University talked about how dioxin exposure increases bone mass and decreases fat cells in bone marrow while also disrupting bone resorption.
• Texas A&M University's Meichen Wang discussed her research to develop a broad-acting sorbent that could be used as a dietary supplement to bind to harmful chemicals in the intestine, preventing their absorption in the body and protecting human health.

Eight Northeast SRP Centers Convene at Regional Meeting

Attendees listened attentively to scientific presentations and had lively question-and-answer sessions at the Northeast SRP Meeting.
(Photo courtesy of Michelle Heacock)

The Northeast Superfund Research Program (SRP) Meeting brought together eight SRP Centers to discuss collaborations and network. Held in Woods Hole, Massachusetts, on March 26 – 27, the meeting included scientific presentations and poster sessions.

While attending the meeting, Heacock toured SRP-funded labs at Woods Hole, which include work on killifish. Here, an SRP trainee tends to fish tanks.
(Photo courtesy of Michelle Heacock)

Grantees discussed wide-ranging topics, including the exposome, big data, fate and transport of contaminants, remediation approaches, and community engagement. The meeting also had a special focus on trainee development and included a poster competition, speed mentoring, and networking events.

SRP Health Scientist Administrator Michelle Heacock, Ph.D., was on hand to meet with grantees and hear about their exciting research. "The Northeast SRP Meeting was a unique opportunity for these Centers to come together and discuss their diverse research in a shared regional context," she noted.

The Boston University, Brown University, Columbia University, Dartmouth College, Massachusetts Institute of Technology, Northeastern University, University of Pennsylvania, and University of Rhode Island SRP Centers participated in the meeting.

Workshop Takes Next Steps with Lancet Commission Report

Landrigan, a former SRP grantee who co-authored the Lancet Commission report with Suk and others, presents at the GBD workshop.
(Photo courtesy of Howard Hu)

Environmental health experts gathered March 1 - 2, 2018, in Seattle, Washington, to create a strategic framework for addressing global pollution using the 2017 Lancet Commission on Pollution and Health report. The workshop was convened by the Global Burden of Disease (GBD) project's Pollution and Health Initiative.

Several current and previous Superfund Research Program (SRP) grantees attended the workshop, including Philippe Grandjean, Ph.D., from the University of Rhode Island SRP Center; Philip Landrigan, M.D., from the Ichan School of Medicine at Mount Sinai; Howard Hu, M.D., from the University of Toronto; and David Bellinger, Ph.D., from Harvard University. Landrigan, one of 40 international authors of the Lancet Commission report, discussed plans to establish a Global Pollution Observatory that will collect and curate data on pollution and pollution-related disease to share with the scientific community.

Hu, another former SRP grantee, organized the GBD workshop.
(Photo courtesy of Howard Hu)

According to the report, 16 percent of all deaths worldwide can be directly linked to pollution, such as air pollution, lead, neurodevelopmental toxicants, and climate change. The economic costs associated with pollution were estimated at more than $4.6 trillion per year.

"We realized that it was important to document not only the health impacts of global pollution, but also the measurable costs of pollution as an economic burden," said SRP Director Bill Suk, Ph.D., an author on the report. "This is the first analysis to comprehensively report both aspects."

The GBD workshop and related efforts are exploring ways to enhance data collection, improve communication with decision-makers, and reduce pollution to decrease the burden of disease and associated economic costs around the world.

Iowa SRP Center Model Enables Accurate Air Pollutant Measurements

Iowa SRP Center researchers use PUF-PAS samplers to measure PCBs in air, including in New Bedford Harbor, shown here.
(Photo courtesy of the Iowa SRP Center)

The University of Iowa Superfund Research Program (SRP) released a Web-based application to help researchers and regulators more accurately determine pollutant concentrations in air using passive air samplers. The application is designed to predict the sampling rates and volumes captured by passive air samplers equipped with polyurethane foam (PUF-PAS), which are frequently used to capture and measure airborne persistent organic pollutants (POPs).

The Web-based application helps users understand how much air went through a PUF-PAS sampler, which is needed to predict the airborne POP concentration. It uses a mathematical model based on user-entered deployment dates of the passive sampler and the compounds of interest. The model uses publicly available hourly meteorological data and the physical and chemical properties of the target compounds to predict the sampling rate and sampling volume for gas-phase compounds captured by the sampler. It can be used to accurately predict the sampling volume of passive air samplers deployed anywhere in the world.

This interface was developed based on research described in an Iowa SRP Center publication led by Keri Hornbuckle, Ph.D. In a 2017 Risk e-Learning webinar, Hornbuckle described this work, as well as other methods for accurate and reproducible measurements of polychlorinated biphenyls (PCBs), a type of POP, in air, water, soil, sediment, pore water, plant tissue, and human blood serum.

SRP Researchers Create Ultrastretchable Nano Barrier for Numerous Applications

Novel textured coatings made from graphene can act as ultrastretchable barriers to stop chemicals and other molecules from passing through, according to new research from the Brown University Superfund Research Program Center. The authors suggest these novel films could be used to create multifunctional fabrics and responsive devices, including personal monitoring equipment, wearable electronics, and soft robotics.

The research team used graphene nano-sheets that can change shape by folding and unfolding to mimic a more elastic behavior, which improves upon traditional approaches that create a material that is quite stiff and cracks easily. The stretchable technology also can function as a sensor to detect certain chemicals and to act as either an impassible or selective barrier. These characteristics will make the material useful for a variety of future cutting-edge applications.

SRP Grantees Work to Improve Water Quality for Native Communities

Lewis pictured in the UNM College of Pharmacy's monthly magazine
(Photo courtesy of the UNM College of Pharmacy's Lobo Script)

Researchers at the University of New Mexico's Superfund Research Program Center (UNM SRP Center) are studying the effects of exposure to uranium and mixed metals mining waste in water on Native Americans in the southwest. Funded in the fall of 2017, their work is already making headlines. Center Director Johnnye Lewis, Ph.D., was recently featured in the UNM College of Pharmacy's Lobo Script monthly magazine and in the Albuquerque Journal.

Lewis described how UNM SRP Center researchers are investigating the health impacts of mixed metals and uranium exposures on Native Americans, how these contaminants move through the environment, and how they can be stabilized to decrease their movement into surface water and to protect human health.

Lewis explained that an estimated 40 percent of surface water in the western U.S. is contaminated with uranium, and tribes in these regions rely much more heavily on surface water than do other populations for not only drinking but also for irrigation, livestock watering, and cultural uses.

Lewis is also part of a collaboration aiming to reach the World Health Organization's goal of providing clean drinking water to all people by 2030. Organized by the United Nations Children's Fund, the Global Water Challenge, Stanford University's Water in the West program, and the U.S. Water Partnership, the collaborative is identifying barriers and developing a roadmap to ensure the U.S. meets the 2030 goal. Lewis' work in this collaborative is focused on providing clean drinking water for Native Americans and others in the southwest, where infrastructure is deteriorating and unaffordable for many communities.

SRP Grantees Participate in Federal PFAS Information Exchange

On February 5 - 6, Superfund Research Program (SRP) grantees provided their expertise and perspectives during the Federal Information Exchange on per- and polyfluoroalkyl substances (PFAS) in Bethesda, Maryland. PFAS chemicals have received increasing attention because they have been found in several drinking water systems and have been linked to reproductive, developmental, liver, kidney, and immunological effects.

Hosted by the Toxics and Risks Subcommittee of the National Science and Technology Council, the workshop provided a forum to share emerging data and key knowledge gaps in the sources, pathways, treatment, and health effects of PFAS. SRP grantees Raymond Ball, Ph.D., Jennifer Guelfo, Ph.D., and Angela Slitt, Ph.D., participated in the workshop.

"The meeting was informative and underlined the magnitude of the PFAS problem," said Ball, president and principal engineer at the NIEHS-funded small business EnChem Engineering. As part of his SRP project, Ball's team is developing a technology to expedite the removal of PFAS from soil and groundwater.

The meeting opened with remarks from senior government officials, including NIEHS Director Linda Birnbaum, Ph.D., and the Centers for Disease Control and Prevention's National Center for Environmental Health Director Patrick Breysse, Ph.D. Following talks from researchers about new findings in their areas of expertise, federal employees and federally funded researchers participated in breakout sessions to discuss current scientific knowledge and future directions.

"The meeting provided a platform for researchers and employees across federal agencies to hear how each is engaged in science and decision-making regarding PFAS," said Guelfo, a researcher at the Brown University SRP Center. Her recent work has focused on using publicly available data to develop models that predict areas with potential PFAS groundwater contamination.

"Given that PFAS includes thousands of compounds, one recurring theme was the need for methods for prioritizing compounds and the need to understand the influence of mixtures," Guelfo added. "There was also a lot of discussion about developing standard methods for PFAS analysis."

In addition to discussions about routes of exposure and treatment methods, time was set aside to discuss the current understanding of the health effects of PFAS. Slitt, a grantee at the University of Rhode Island SRP Center, is studying whether PFAS exposure increases the risk for obesity-induced fatty liver disease and metabolic disorders.

In the final session, participants discussed risk assessment, consideration of data needs for protecting human health, and ongoing coordination and communication across federal agencies. The workshop was immediately followed by a closed Toxics and Risks Subcommittee meeting to discuss how these findings will inform agencies moving forward.

Improving Site Characterization to Assess Contaminant Removal

Brusseau is a professor in the School of Earth and Environmental Sciences at the University of Arizona.
(Photo courtesy of Mark Brusseau)

A computational model can be used to measure how different factors influence the removal of groundwater contaminants at hazardous waste sites, according to a study from the University of Arizona Superfund Research Program Center. Researchers led by Mark Brusseau, Ph.D., developed the predictive model and found that their contaminant estimates from the model compared well to measurements taken at a Superfund site.

Their model uses the relationship between reductions in contaminant discharge and removal as the metric to examine remediation efficiency. Characterization methods such as this may help researchers more easily understand factors that may impact the distribution of contaminants at a site, which can provide more information about the effectiveness of remediation efforts.

Building on these findings, the research team used the model to examine factors that influence contaminant removal in large groundwater contaminant plumes. Specifically, they looked at areas with low groundwater flow adjacent to large aquifer systems, which is common at many hazardous waste sites.

They found that the location of pump-and-treat wells, relative to contaminated water, can have a significant impact on how effectively contaminants are removed and how they persist in large groundwater systems. Based on well configuration, zones may be formed where contaminants remain stagnant, which reduces the effectiveness of pump and treat. This illustrates the need for dynamic system operations in which the system is routinely monitored and operational conditions are modified to maintain peak performance.

In a 2017 Risk e-Learning webinar, Brusseau described this work, as well as other efforts to improve characterization methods to understand the factors contributing to the persistence of contaminants in groundwater.

SRP Research Finds Ancestry-Based Differences in Telomere Length Genes

People with different ancestries may inherit telomere length differently, according to a new study from the Columbia University Superfund Research Program (SRP) Center. Telomeres are segments at the end of DNA, and telomere length plays an important role in aging and aging-related diseases. This study provides new information about the genes associated with telomere length across populations and highlights the importance of including diverse populations in genome-wide association studies (GWAS).

Although telomere length is a heritable trait, it still can vary considerably between individuals and populations, leading scientists to study how it is passed between generations. Previous studies that have investigated how telomere length is inherited have relied primarily on populations of European descent. In this study, researchers used samples from the Health Effects of Arsenic Longitudinal Study cohort in Bangladesh and found important ancestry-based differences in how telomere length might be passed on.

In addition to replicating some findings from other genome-wide association studies, such as those showing that gene regions called TERT and TERC are related to telomere length, the researchers also identified a new association. The study reports a link between telomere length and a gene region called RTEL1 and, importantly, a distinct second region of RTEL1 that had not been previously identified in relation to telomere length. This specific region of RTEL1 is common in South Asian populations but less so in other populations.

SRP Grantee Featured in Science Friday Video

Karletta Chief, Ph.D., of the University of Arizona Superfund Research Program (SRP) Center was recently interviewed on Science Friday. Her interview coincided with the release of the sixth and final installment of a short video anthology, "Breakthrough: Portraits of Women in Science," which follows women working at the forefront of their fields.

Bitter Water, the last film in the anthology, featured Chief and her SRP-funded work to address the concerns of the Navajo community after the Gold King Mine spill in 2015. In both the video and the interview, Chief explained how her research team helped identify more than 100 unique cultural uses of the river that were not previously considered in exposure estimates. They also studied how metals and other potentially toxic contaminants moved through the river’s sediment and water. Chief further shared her personal history and experiences leading her to become a hydrologist.

The Breakthrough anthology from Science Friday and the Howard Hughes Medical Institute blends the personal stories of women in science, technology, engineering, and math with highlights of their innovative scientific research and accomplishments.

Chief collects a sediment core sample from the river to measure the concentration of various contaminants after the mine spill.
(Photo courtesy of Science Friday)

Fish Adaptation to TCDD Seen at the Genome Level

Long-term exposure to environmental toxicants can affect the genome of Hudson River tomcod much more than previously expected, according to researchers led by Isaac Wirgin, Ph.D., at the New York University School of Medicine. A recent study led by Isaac Wirgin, Ph.D. and his team sheds light on the effects of exposure to the pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the genomes, or full set of genes, from two tomcod populations 86 miles apart with distinctly different exposure histories.

A mature Atlantic tomcod collected from the Hudson River.
(Photo courtesy of Mark Mattson, Normandeau Associates via Science/AAAS)

The recent findings build on Wirgin’s Superfund Research Program (SRP)-funded work. The Hudson River Estuary in the New York City Metropolitan area has a long history of pollutants from both local and distant sources. In a study in the journal Science in 2011, funded in part by the SRP, Wirgin and his team describe how Atlantic tomcod in the Hudson River have genetically evolved to withstand the effects of pollutants, primarily polychlorinated biphenyls (PCBs), in the river.

In the new study, the researchers used larvae from both the pollutant-resistant Hudson River tomcod population and a non-resistant nearby Shinnecock Bay population. They exposed the two groups of larvae to TCDD and then compared the global expression of genes in the resistant and non-resistant tomcod.

They observed differences between the two populations in the number of genes that were expressed differently at all doses of TCDD. At the two lowest TCDD doses, 250 and 1,141 genes were differentially expressed in Shinnecock Bay larvae compared with only 14 and 12, respectively, in Hudson River larvae. At the highest dose, 934 genes were differentially expressed in Shinnecock Bay larvae and 173 in Hudson River larvae, but only 16 percent of affected genes were shared among both populations.

“I think the data is pretty dramatic in demonstrating just how great the effect of Hudson River-borne pollutants has been on the evolution of its tomcod population,” said Wirgin. “Pollutants can have far greater effects on exposed populations than we ever imagined.”

According to the authors, understanding differences in gene expression will allow them to better understand the toxic impacts of exposure and evaluate their mechanistic basis.

SRP Grantee Takes Cleanup Technology to the Field

Superfund Research Program (SRP) grantee Dibakar Bhattacharyya, Ph.D., of the University of Kentucky was recently awarded funding to help the Chevron Corporation remove metals and other potentially harmful contaminants from wastewater created during oil production.

Bhattacharyya's SRP-funded work was critical in laying the foundation that provided the opportunity to transfer his technology from the laboratory to the field. His team at the University of Kentucky SRP Center pioneered the development and use of specialized membranes to break down toxic organic compounds, such as polychlorinated biphenyls, trichloroethylene, and napthenic acids in water.

These membranes, which are embedded with nanoparticles in the lab, also can be used to capture metals like arsenic and mercury, making them ideal for the work with Chevron.