Design, Results, and Conclusions
Biomarker validation criterion:
A significant effect by oxidative insult should be measured at two doses and at more than one time point.
Markers did not meet criteria in all three models
Misinterpretation of the BOSS best biomarker: F2-isoprostanes
Distinguishing chemical from enzymatic F2-isoprostanegeneration by 8-iso-PGF2α/ PGF2αRatio
BOSS Major Conclusions
- Distinction between chemical and enzymatic lipid peroxidation is essential for the correct interpretation of oxidative stress.
- Measuring only 8-iso-PGF2α is no longer sufficient to identify oxidative stress due to confounding generation by prostaglandin synthases.
- The 8-iso-PGF2α / PGF2α ratio distinguishes between F2-isoprostane generation by oxidative stress or inflammation in vitro, in vivo, and in humans.
- Literature studies identifying oxidative stress by increased 8-iso-PGF2α alone could have been misinterpreted and therefore require reexamination and most likely new interpretation.