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Equipment

Immunohistochemistry

National Toxicology Program
National Toxicology Program (NTP) Data and Reports Website

The Immunohistochemistry (IHC) Laboratory utilizes two automated staining systems:

  • The flexible Biocare intelliPATH™ FLX is an open platform system capable of supporting multiple users and staining 50 slides simultaneously.
  • The fully automated Ventana Discovery™ XT is a start to finish system capable of staining 30 slides at a time.

The Laboratory uses modern procedures for standardized antigen retrieval, including the high capacity Biocare Decloaking Chamber™ NxGen.

A Leica CM3050S cryostat is used for sectioning frozen tissues.

A Reichert-Jung 2030 microtome is used for sectioning in-house control tissues.

The Laboratory also has a 10-head Olympus BX51 microscope with large screen viewing and image capture capabilities, and an Olympus BX61 microscope with motorized stage for viewing and capturing fluorescent stains.

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