New Zebrafish Line for Studying Aryl Hydrocarbon Receptor
Robert L. Tanguay, Britton C. Goodale
Oregon State University
NIEHS grants P30ES000210, P42ES016465
NIEHS-supported researchers have developed a new zebrafish line that expresses a non-functioning aryl hydrocarbon receptor (AHR)2. AHR2 mediates the toxic effects of dioxin, and a functional knockout line for this receptor could help reveal more about its role in toxicological response and other biological processes.
Zebrafish offer a useful model for studying the effects of exposures on development. They have AHR2, AHR1A, and AHR1B versions of the receptor. The researchers used a method called targeting induced local lesions in genomes (TILLING) to create a mutation in the ahr2 gene (ahr2(hu3335)) and then confirmed the absence of AHR2 activity in the resulting mutant line. With the new zebrafish line, investigators can study AHR2 function throughout the organism’s lifespans or examine the function of AHR1A and AHR1B, which has been difficult to do.
Citation: Goodale BC, La Du JK, Bisson WH, Janszen DB, Waters KM, Tanguay RL. 2012. AHR2 mutant reveals functional diversity of aryl hydrocarbon receptors in zebrafish. PLoS One 7(1):e29346.
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