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Degenerative Lesions of the Liver

The Digitized Atlas of Mouse Liver Lesions

Amyloid Deposition - Click on thumbnails to view larger images
Amyloid deposition is rare in B6C3F1 mice but more common in the liver (and also the gallbladder) of CD-1 mice. It is usually located beneath the sinusoidal lining and in the wall of portal blood vessels. Amyloid deposition around blood vessels in the portal areas.
amyloid deposition in mouse liver
amyloid deposition in mouse liver
amyloid deposition in mouse liver
Hepatocyte Atrophy - Click on thumbnails to view larger images
Hepatocyte atrophy and glycogen depletion after 90 days of exposure to malonaldehyde. Note paucity of cytoplasm and crowding of nuclei.
hepatocyte atrophy in mouse liver
Bile Duct Cyst - Click on thumbnails to view larger images
Bile duct cyst. A solitary biliary cyst at the edge of a liver lobe. This was grossly visible and such cysts are typically filled with clear to straw colored fluid and lined by flattened biliary epithelium. They are found occasionally in the liver of untreated mice. Note: The clear space in the cytoplasm of the hepatocytes was previously occupied by glycogen which has dissolved out in the aqueous formalin fixative.
bile duct cyst
bile duct cyst
bile duct cyst
Cystic Degeneration (Spongiosis hepatis) - Click on thumbnails to view larger images
Cystic degeneration is not as common in mice as in rats. It consists of multilocular cyst-like structures containing granular or flocculent eosinophilic material and, occasionally, erythrocytes. It is believed to represent a degenerative change in Ito cells (stellate cells) and may be seen in normal hepatic parenchyma as well as in proliferative hepatocellular lesions such as foci and neoplasms.
cystic degeneration in liver
Fatty Change (Fatty Metamorphosis) - Click on thumbnails to view larger images
Fatty change, mild. The circular clear spaces represent areas previously occupied by fat in this mouse on a choline deficient diet. The fat is dissolved out by xylene during processing of the tissue.
fatty change in liver
fatty change in liver
fatty change in liver
Periportal fatty change. The large, sharply delineated, clear vacuoles represent fat that has been dissolved during tissue processing. Deposition of fat in the hepatocyte cytoplasm often displaces the hepatocyte nucleus to the periphery of the cell.
periportal fatty change in liver
periportal fatty change in liver
 
Large areas of fatty change without a distinct lobular pattern are present in this liver. The large, sharply delineated, clear vacuoles represent macrovesicular fat that has been dissolved during tissue processing. Deposition of fat in the hepatocyte cytoplasm often displaces the hepatocyte nucleus to the periphery of the cell.
large areas of fatty change liver
large areas of fatty change liver
large areas of fatty change liver
This represents an example of microvesicular fatty change. Multiple small vacuoles present within the hepatocytes give the cytoplasm a foamy appearance.
localized area of hepatocytes
localized area of hepatocytes
 
This example of fatty change represents a combination of microvesicular and macrovesicular fatty change with a distinct lobular pattern. The macrovesicular fat is localized at the periphery and probably represents condensation of microvesicular vacuoles.
fatty change in liver
fatty change in liver
 
This is an example of focal fatty change with affected hepatocytes containing a mixture of macrovesicular and microvesicular fat.
fatty change in liver
fatty change in liver
 
A specific example of focal fatty change seen in mice and present in the median lobe represents tension lipidosis when it is near the falciform ligament and gallbladder.
fatty change in liver
fatty change in liver
 
Fatty change (small lipid droplets) in the liver of a mouse given ethionine.
fatty change in liver
Cytoplasmic vacuolation of hepatocytes (fat) in a mouse given benzene hexachloride. Vacuoles vary from small globules to large vacuolated areas in the cytoplasm.
fatty change in liver
Glycogen Deposition - Click on thumbnails to view larger images
Centrilobular deposition of glycogen in a B6C3F1 mouse.
glycogen deposition in mouse liver
Glycogen rich hepatocytes are localized in periportal and mid-lobular areas of the liver of this untreated male B6C3F1 mouse.
glycogen rich hepatocytes
glycogen rich hepatocytes
glycogen rich hepatocytes
Periodic acid Schiff stain of glycogen in a formalin fixed liver without diastase treatment on the left and with diastase treatment on the right.
Periodic acid Schiff stain
Periodic acid Schiff stain for glycogen in an alcoholic formalin fixed liver. Treatment with diastase on the left and without diastase on the right.
Periodic acid Schiff stain
Periodic acid Schiff stain of glucose-6-phosphatase +/+ mouse (left) and -/- mouse (right) showing glycogen storage in hepatocytes of formalin fixed liver.
Periodic acid Schiff stain
Hyaline Change - Click on thumbnails to view larger images
Centrilobular hepatocytes have alterations in their cytoplasm characterized by increased eosinophilia with a fine to coarse flocculent texture. In some situation the appearance may be more homogeneous. The terminology that might be used for this change includes hyaline change, hyaline degeneration, or cytoplasmic alteration. If the latter term is used, the change must be adequately described in a pathology narrative since the term cytoplasmic alteration has different connotations to different individuals.
hyaline change
hyaline change
 
Hyaline Bodies - Click on thumbnails to view larger images
Hyaline bodies are occasionally seen in liver degeneration as well as in hepatocellular neoplasms of mice. In this example a large number of large hyaline bodies are concentrated in one region of the liver.
hyaline bodies
hyaline bodies
hyaline bodies

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