Instrument Cleaning - Rnase Decontamination
RNase Decontamination Solution
Ambion - The RNA Comapny
BackgroundRNaseZap® completely removes RNase contamination from glass and plastic surfaces. It contains three different ingredients known to be active against RNase. It effectively removes high levels of RNase contamination that similar products cannot. RNaseZap has been used to remove RNase contamination from reaction vessels. With thorough rinsing, RNaseZap leaves no residues that are inhibitory to enzymatic reactions.
Instructions for UseRNaseZap is ready to use
Do not dilute because dilution will reduce its effectiveness. If there is a precipitate (as may happen at low temperatures), shake and/or heat at 37˚C to bring the precipitate back into solution.
Always wear gloves while using RNaseZap because prolonged contact with skin may cause irritation. When using the spray bottle attachment, always work in a fume hood as inhalation of aerosol or vapor may cause irritation to lungs and mucous membranes.
Please note that RNaseZap should not be used on corrodible metal surfaces.
For cleaning work surfaces:
Apply RNaseZap directly to surface to be cleaned, wipe thoroughly with paper towel, rinse with water and then dry with clean paper towel.
For cleaning lab apparatus:
Apply RNaseZap liberally to a paper towel and wipe all exposed surfaces of the apparatus thoroughly. Rinse with water and then wipe dry. Some small parts may be cleaned by briefly soaking them in RNaseZap, rinsing them with water and then drying.
For cleaning plastic and glass vessels:
Add or spray enough RNaseZap so that the entire surface of the vessel can be coated with the solution upon swirling, or vortexing in the case of centrifuge and microfuge tubes. After discarding the solution, rinse vessels thoroughly two times with distilled water.
For cleaning pipettors:
Remove shaft from pipettor according to manufacturers instructions. Remove seals and gaskets from the shaft and then soak shaft for one minute in RNaseZap. Rinse the shaft thoroughly with water and then reassemble pipettor.
RNaseZap SpecificationsStorage and Stability:
Store RNaseZap at room temperature.
RNaseZap is stable for 6 months from the date received.
If contact with eyes occurs, immediately flush with water and call physician. If swallowed, do not induce vomiting. Give plenty of water and contact physician. If inhaled, remove to a well ventilated area. For more information, see the MSDS that follows.
RNaseZap Material Safety Data Sheet
Appearance and odor: clear liquid
pH: 7–7.4 (by pH paper)
Boiling point: n/a
Specific gravity: 1.0 (equal to water)
Solubility in H2O: Completely soluble
Fire and explosion hazard data
Flash point: >212˚F
Flammable limits in air: >212˚F
Extinguishing media: Contact with combustible material may cause fire. Use media suitable to extinguish surrounding fire such as water, CO2, or foam.
Special fire fighting: Wear self-contained breathing apparatus and protective clothing.
Unusual Fire/explosion hazards: n/a
Health hazard data
Effects of overexposure: Irritating to eyes, respiratory system and skin, readily absorbed into the skin WEAR GLOVES AT ALL TIMES.
Emergency first aid: Wash affected area with copious amounts of water. Irrigate eyes for ≥15 min. See physician, treat symptomatically.
Incompatibility: Strong oxidizing agents, reducing agents and acids & bases.
Hazardous Decomp. Products: Toxic fumes of CO, CO2.
Hazardous Polymerization: Will not occur.
Spill or leak procedures
If released or spilled: Absorb on sand and place in open container for disposal. Ventilate area & wash spill site.
Waste disposal method: Dispose of according to federal, local and state regulations.
Special protection and precaution information
Respiratory protection: Use NIOSH approved respirator as needed.
Ventilation: Use lab hood as appropriate.
Protective gloves: Neoprene or PVC
Eye protection: Use full eye protection-goggles as a standard precautionary measure.
Handling procedures: Follow routine safe handling procedures.
This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are offered solely for your consideration, investigation, and verification. These suggestions should not be confused with either state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of these data and information must be determined by the user to be in accordance with applicable federal, state, and local regulations.