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National Institute of Environmental Health SciencesNational Institutes of Health

Assays

Assays for possible markers of oxidative stress measurement include assays that measure oxidation of lipids, proteins, DNA and a group of antioxidants.

  • Lipid peroxidation assays: lipid hydroperoxides, TBARS, MDA, isoprostanes, various HETEs.

  • Protein oxidation assays: protein carbonyls, various tyrosine products, methionine sulfoxidation.

  • DNA oxidation assays: 8-OH-dG, oxidation changes by the Comet assay, M1G.

  • Antioxidants: Ascorbic acid, tocopherols, GSH, GSSG, uric acid, TAC (total antioxidant capacity)
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Last Reviewed: August 17, 2007