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Research Methods

Clinical Investigation of Host Defense Group

Protein Discovery in Innate Immunity Signaling

 

  • Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
  • Affinity precipitation and immunoprecipitation
  • Immobilized metal affinity chromatography
  • Tandem mass spectrometry
  • Two-Dimensional Polyacrylamide Gel Electrophoresis

 

Role of Cholesterol Trafficking and Lipid Rafts in Innate Immunity

 

  • Raft isolation
  • Confocal microscopy
  • RNA interference
  • Cholesterol quantitation
  • Rho GTPase assays
  • Mouse models
  • Macrophage and neutrophil isolation

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