Various Resources

Forms

• LMC Special Volunteer or Guest Researcher Appt. Checklist (New/Renewal) - Non-U.S. Citizen   Word  (33KB) , PDF  (25KB)
• LMC Post-Doc IRTA, Appt. Renewal Checklist   Word  (32KB) , PDF  (25KB)
• LMC Post-Doc IRTA, New Appt. Checklist Word  (32KB) , PDF  (23KB)
• LMC Post-Doc Visiting Fellow, Appt. Renewal Checklist,   Word  (49KB) , PDF  (178KB)
• LMC Post-Doc Visiting Fellow, New Appt. Checklist   Word  (44KB) , PDF  (111KB)
• LMC Research Fellow (VP), New Appt. Checklist   Word  (51KB) , PDF  (147KB)
• LMC Research Fellow (VP), Appt. Renewal Checklist   Word  (49KB) , PDF  (178KB)
• LMC Special Volunteer or Guest Researcher Appt. Checklist (New/Renewal) - U.S. Citizen Word  (33KB) , PDF  (24KB)

Special Recognitions

Years of Government Service

The LMC wishes to congratulate two of its members on being recognized for service to the Institute. Rob Wine (left picture) and Tom Eling (right picture) of LMC were presented with certificates by Dr. Linda Birnbaum, Director of the NIEHS, for years of government service, with Rob being recognized for 20 years, and Tom being recognized for an amazing 40 years of service to NIEHS.

Lai Gives NIH Women Scientists Advisor (WSA) Scholar Seminar

Anne Lai, Ph.D., has been selected to give an oral presentation at the second Annual NIH Women Scientists Advisor (WSA) Scholar Seminar on Friday, March 2, 2012 at Wilson Hall on the Bethesda campus. She is one of three women scientists chosen from a pool of all of the 2012 female Fellow Award for Research Excellence (FARE) winners, and will present 'Dynamics of DNA methylation during B lymphocyte activation and differentiation.' Lai is a postdoctoral fellow in the Eukaryotic Transcriptional Regulation Group led by Paul Wade, Ph.D.

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Available Techniques & Expertise in LMC

Sequencing Lab Group (SLG) Mission

The mission of the Automated DNA Sequencing Core Unit is to provide excellent quality data in a reasonable amount of time. Our policy is to provide each investigator an equal opportunity to submit samples and our goal is to return analyzed data within 1 to 2 days from the samples submission. Our average turnaround time is less than 24 hours from sample submission. The NIEHS DNA Sequencing Core uses biochemical methods to determine the order of nucleotide bases - adenine, guanine, cytosine and thymine - in a DNA sample. The core uses Perkin-Elmer ABI Model 3100 and Model 3130 sequencers, which offer a variety of services including single pass primer extension using plasmid DNA, PCR products or genomic DNA as a sequencing template. The single pass extension using conventional sequencing primers such as M13, T7, T3 and SP6 or a custom primer, can provide 600-650 base pairs of "good" or viable sequence data with a quick turnaround time. Shotgun sequencing strategies can be used to sequence a variety of samples including cosmids, BACs and PACs.

The DNA Sequencing Core does not accept samples from the general public, but any investigator from within the Institute may submit samples for analysis at no cost. Greg Solomon manages the facility, with biologist Jason Malphurs and sequencing engineer John Otstot assisting in the generation of sequence data.
Sequencing Lab Group (SLG) Publications  (20KB)

Sequencing Lab Group (SLG) Staff

Greg Solomon
Manager

Tel (919) 541-1812
solomon1@niehs.nih.gov

Jason Malphurs
Biologist

Tel (919) 316-4588
malphurs@niehs.nih.gov

John Otstot
Biologist

Tel (919) 541-0493
otstot@niehs.nih.gov

In Vivo Imaging System for Bioluminescence and Fluorescence

Bioluminescent Images of Tumor-bearing Mice
Human breast carcinoma cells expressing firefly luciferase were injected into the mammary fat pads of nude (immunocompromised) female mice. To visualize the location of tumor cells, mice were injected intraperitoneally with luciferin, then anesthetized and placed in the Caliper Spectrum in vivo imaging system at NIEHS. Each image shows an overlay of the bioluminescent signal on a white light image of the mouse (ventral view).
A) Mouse showing bioluminescent signal from the primary tumor in the mammary fat pad 6 weeks after injection of tumor cells. Note the scale indicates the most intense pixels represent approximately 1 x 109 photons/sec/cm2.
B) Same mouse, imaged one week after surgical resection of the primary tumor, showing three areas of bioluminescence: 1) potential remaining cells from primary tumor, or metastasis to adjacent fat pad; 2) potential metastasis to another mammary fat pad or axillary lymph nodes; and 3) potential lung metastasis. Maximal bioluminescent signal in this mouse was approximately 1 x 104 photons/sec/cm2 which would not have been visible when the primary tumor was present.
C) Another mouse imaged one week after the primary tumor was surgically resected. Note the absence of tumor over the mammary fat pad, but the appearance of bioluminescence over the chest. The most intense signal from this apparent lung metastasis represents approximately 4 x 104 photons/sec/cm2. (D. Ray, P. Myers, and J. Roberts)
Contact John Roberts in LMC for information and training in the use of the Caliper Spectrum IVIS located within the animal facility at NIEHS.

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Calendar of Events

For more on the LMC Journal Club and Seminar Series, see below.

Journal Club Mission

The LMC journal club has been established to foster scientific discussion on the most recent science publications of general interest. All members are invited to pick, present and lead a discussion on a recent journal article.

Notes to Participants

All meetings will be held at 12:00 noon unless notified otherwise. Anyone in the LMC is welcome to present, including fellows, biologists and principal investigators. Anyone interested in leading a discussion should contact Jennifer Sims at (919) 541-1101 or by email at simsj2@niehs.nih.gov .

Some Guidelines

We ask that a PDF file of the article to be discussed be emailed to the organizer along with a brief 2-3 sentence summary for distribution at least 7 days prior to leading the meeting. We also ask if a speaker must cancel their discussion that 1 week notice be given so that the organizer can locate a substitute or rearrange the schedule.

Please be aware the schedule is subject to change. Scheduled dates as well as speaker will be updated on the Web site so stay tuned for journal club news.

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Seminar Series Schedule

Laboratory of Molecular Carcinogenesis

2012 - Thursdays, 10:00 A.M., D450 Conference Room

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