Diane L. Spencer, M.S.
Report on Carcinogens
Diane L. Spencer, M.S.
Tel (919) 541-2759
P.O. Box 12233
Mail Drop K2-14
Research Triangle Park, North Carolina 27709
Diane L. Spencer, M.S., is a Health Scientist in the Office of the Report on Carcinogens (RoC), where she serves as a scientific resource. She joined the RoC team in December 2008 after working for more than five years as a biologist in the NTP Center for the Evaluation of Risks to Human Reproduction (CERHR), providing scientific, administrative and technical support. Prior to Spencer's work at CERHR, she worked for six months in the NTP Office of Liaison, Policy and Review, helping to coordinate scientific workshops and exhibitions for the NTP. Spencer has been employed at the NIEHS since 1984, initially as a laboratory technician in the Laboratory of Environmental Carcinogenesis and Mutagenesis, and later as a researcher and laboratory manager in the Laboratory of Computational Biology and Risk Analysis.
Research interests in the laboratory included projects assessing human risk associated with dioxin (TCDD) exposure. She developed an assay using human peripheral blood lymphocytes to measure constitutive and TCDD-induced expression of CYP1B1 using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Earlier laboratory research involved using cultured mammalian cell lines and molecular biology techniques to investigate mechanisms of mutagenesis, with specific emphasis on mutation rates and "epigenetic" mechanisms of mutation induction.
A charter (1982) member of the Genetics and Environmental Mutagen Society, Spencer has served as Secretary, Treasurer, President-Elect, and President, and continues to be an active member of the Society.
Spencer, a native of Canada, earned a B.A. in biology from Rollins College in Winter Park, Florida and an M.S. in biology from the University of Miami in Coral Gables, Florida.
- Spencer DL, Masten SA, Lanier KM, Yang XP, Grassman JA, Miller CR, Sutter TR, Lucier GW, and Walker NJ (1999): Quantitative analysis of constitutive and 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cytochrome P450 1B1 expression in human lymphocytes. Cancer Epidemiology, Biomarkers & Prevention 8(2):139-146. [Abstract (http://preview.ncbi.nlm.nih.gov/pubmed/10067811?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=6) ]
- Liechty MC, Scalzi JM, Sims KR, Crosby HC Jr., Spencer DL, Davis LM, Caspary WJ, and Hozier JC (1998): Analysis of large and small colony L5178Y tk (-/-) mouse lymphoma mutants by loss of heterozygosity (LOH) and by whole chromosome 11 painting: detection of recombination. Mutagenesis 13(5):461-474. [Abstract (http://preview.ncbi.nlm.nih.gov/pubmed/9800191?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=7) ]
- Masten SA, Grassman JA, Yang X, Miller CR, Spencer DL, Lanier KM, Walker NJ, Jung D, Konietzko J, Edler L, Patterson Jr. DG, Needham LL, and Lucier GW (1997): Mechanistically based markers of exposure and response to dioxin in occupationally exposed individuals. Organohalogen Compounds 34:80-85.
- Grassman J, Clark G, Yang X, Masten S, Spencer D, Landi MT, Miller C, Walker NJ, and Lucier GW (1996): Estimating variability in response to dioxin after exposure in Seveso, Italy. Organohalogen Compounds 30:302-307.
- Spencer DL, Caspary WJ, Hines KC and Tindall KR (1996): 5-Azacytidine-induced 6-thioguanine resistance at the gpt locus in AS52 cells: cellular response. Environmental and Molecular Mutagenesis 28(2):100-106. [Abstract (http://preview.ncbi.nlm.nih.gov/pubmed/8844990?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=10) ]
- Spencer DL, Hines KC and Caspary WJ (1994): An in situ protocol for measuring the expression of chemically-induced mutations in mammalian cells. Mutation Research 312(2):85-97. [Abstract (http://preview.ncbi.nlm.nih.gov/pubmed/7510835?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=14) ]
- Spencer DL and Caspary WJ (1994): In situ and suspension protocols for chemically-induced mutation at the tk locus in L5178Y MOLY cells: dose response and colony size distribution. Mutation Research 322(4):291-300. [Abstract (http://preview.ncbi.nlm.nih.gov/pubmed/7523923?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=12) ]
- Stopper H, Körber C, Spencer DL, Kirchner S, Caspary WJ, and Schiffmann D (1993): An investigation of micronucleus and mutation induction by oxazepam in mammalian cells. Mutagenesis 8(5):449-455. [Abstract (http://preview.ncbi.nlm.nih.gov/pubmed/8231827?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=15) ]
- Rudd CJ, Daston DS and Caspary WJ (1990): Spontaneous mutation rates in mammalian cells: effect of differential growth rates and phenotypic lag. Genetics 126(2):435-442. [Abstract (http://preview.ncbi.nlm.nih.gov/pubmed/2123164?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=2) ]
- Caspary WJ, Daston DS, Myhr BC, Mitchell AD, Rudd CJ, and Lee PS (1988): Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: interlaboratory reproducibility and assessment. Environmental and Molecular Mutagenesis 12 (Suppl. 13):195-229. [Abstract (http://preview.ncbi.nlm.nih.gov/pubmed/3416840?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=4) ]