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Vector Table

Protein Expression

Table Legend

hPlasmidh hSystemh hPromotorh hN-tagh hC-tagh Selection Markerh
  E. coli        
pDest14   T7     Amp
pDest15   T7 GST   Amp
pDest17   T7 His   Amp
pDest24   T7   GST Amp
pET-Dest42   T7   V5-His Amp
pBadDest49   AraBad TrxHis   Amp
pDest526   T7 His-BAP   Amp
pDest527   T7 His   Amp
pDest533   T7 His-KSI   Amp
pDest535   T7 CBP   Amp
pDest544   T7 His-NusA   Amp
pDest546   T7 His-Trx   Amp
pDest565   T7 His-GST   Amp
pDest566   T7 His-MBP   Amp
pDest569   T7 BAP   Amp
pDest575   Tac GST-thrm   Amp
pDest596   Tac MBP   Amp
pDest597   T7 MBP-His   Amp
pET104-Dest   T7 BioEase   Amp
           
  Insect cell        
pDest8 baculovirus polyhedron     Amp
pDest10 baculovirus polyhedron His   Amp
pDest20 baculovirus polyhedron GST   Amp
pDest602 baculovirus polyhedron MBP   Amp/Geneticin
    p10 eGFP marker    
pDest603 baculovirus polyhedron His-GST   Amp/Geneticin
    p10 eGFP marker    
pDeat605 baculovirus polyhedron His   Amp/Geneticin
    p10 eGFP marker    
pDest606 baculovirus polyhedron His-MBP   Amp/Geneticin
    p10 eGFP marker    
pMT-Dest48 stable metallothionine   V5-His Amp/DES Expression
pIB/V5-His-DEST stable OpIE2   V5-His Amp/Blasticidin
pMT-BioEase stable metallothionine BioEase   Amp/DES Expression
           
  Mammalian cell        
pDest26   CMV His   Amp/Geneticin
pDest27   CMV GST   Amp/Geneticin
pTRex-Dest30   CMV-Tet     Amp/Geneticin
pTRex-Dest31   CMV-Tet His   Amp/Geneticin
pcDNA-Dest53   CMV & T7 GFP   Amp/Geneticin
pcDNA3.1/nV5-Dest   CMV & T7 V5   Amp/Geneticin
pcDNA6.2/V5-Dest   CMV & T7 V5   Amp/Blasticidin
pcDNA-Dest47   CMV & T7   GFP Amp/Geneticin
pEF-Dest51   EF-1alpha & T7   V5-His Amp/Blasticidin
pcDNA6 BioEase-Dest   CMV & T7 BAP   Amp/Blasticidin
pDest501   CMV eCFP   Amp
pDest503   CMV   eCFP Amp/Zeocin
pDest515   CMV Flag   Amp
           
  invitro        
pEXP1-Dest   T7 His   EK cleavage
pEXP2-Dest   T7   V5-His  

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Legend

BAP = Biotin Acceptor peptide, Avidity
BioEase = biotinyl tag-Use strep Avidin purification, Invitrogen
CBP = Calmoduline binding protein, Stratagene
eCFP = enhanced Cyan Fluorescent Protein
eGFP = enhanced Green Fluorescent Protein, BD Biosciences
EK = Enterokinase
Flag = Peptide Epitope tag MDYKDDDDK
Geneticin = Neomycin
GFP = Green Fluorescent Protein, Invitrogen
GST = Glutathione S-Transferase
His = 6 x His repeat
KSI = Ketosteroid Isomerase, Novagen
MBP = Maltose Binding Protein
NusA = N-utilyzing substance A protein
thrm = Thrombin cleavage site
Trx = Thioredoxin
TrxHis = Thioredoxin modified to contain His patch when folded properly
V5P = Peptide Epitope Tag GKPIPNPLLGLDST

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Last Reviewed: 17 May 2007